CORE-- Neuroanatomy-Imaging

根据审查小组的要求，定量光显微（LM）密度测定法的使用已从神经解剖成像核心中删除。此外，还做了一些修改，反映了项目建议的变化和项目4的删除。具体来说，增加了LM和EM放射自显影、DiA顺行示踪和TUNEL标记技术，删除了细胞旁标记、生物细胞素顺行示踪和LM重建技术。在抗血清中也有详细的变化：描述了针对黄体酮受体的抗血清，α -肾上腺素能

As requested by the review team, the use of quantitative light microscopic (LM) densitometry has been removed from the Neuroanatomy-lmaging Core. In addition, changes have been made that reflect changes in the project proposals and the deletion of Project 4. Specifically, LM and EM autoradiography, DiA anterograde tract-tracing, and TUNEL labeling techniques have been added, and juxtacellular labeling, biocytin anterograde tract-tracing, and LM reconstruction techniques have been deleted. There also have been changes in the antisera that are detailed: descriptions of antisera raised against the progesterone receptor, the alpha2A-adrenergic receptor and p22-phox are now included, while antisera raised against 5HT receptors, c-Fos, nNOS and vasopressin have been deleted. There have also been changes in the percent utilization of the Neuroanatomy-lmaging Core by the projects reflecting the deletion of Project 4. The revised description of the core is provided below. Major changes are indicated by a line in the left margin. Overall, the Budget has been reduced. A detailed account of the budgetary changes has been included in the Budget Justification.