GEISHA, a Chicken Embryo Gene Expression Resource

描述（由申请人提供）：随着人类基因组测序的完成，人们的注意力集中在了解基因功能上，大约一半的基因仍然未知。关键的第一步是确定基因表达的空间和时间模式。这对于胚胎发生的早期阶段尤其重要，此时发生许多重要的发育过程，并且表达对正常发育至关重要的基因。由于人类胚胎中的基因表达分析在伦理上是站不住脚的，因此必须在模式生物中完成表达模式的绘制。鸡是首要的非哺乳动物模式生物，并用于广泛的研究学科的研究。鸡胚为大规模表达筛选提供了许多优势，包括与人的惊人相似性，低成本和容易获得。 本研究的目的是建立鸡胚原位杂交表达资源。目标如下：1）扩大现有的基因表达筛选工作，称为GEISHA（Gallus EST原位杂交分析），使用自动化高通量整体原位杂交分析来绘制经过四天发育的鸡胚中表达序列的位置; 2）通过平行协调的筛选努力，从个体研究者和从公开的结果获得额外的表达数据; 3）将表达信息存储在关系数据库中，并使用可自由访问的网络界面将表达数据呈现给研究团体。一个鸡解剖本体将被创建来描述表达模式，并使时空查询，数据将被用来注释鸡基因组。这个集中的原位杂交基因表达信息库将为生物医学研究界提供一个有价值的和全面的资源。

DESCRIPTION (provided by applicant): With sequencing of the human genome completed, attention is focusing on understanding gene function, which for approximately half of all genes remains unknown. A crucial first step is to determine spatial and temporal patterns of gene expression. This is especially important for early stages of embryogenesis, when many important developmental processes occur and when genes critical for normal development are expressed. Since gene expression analysis in human embryos is ethically untenable, mapping of expression patterns must be accomplished in model organisms. Chicken is the premiere non-mammalian model organism, and is used in research across a broad spectrum of research disciplines. The chicken embryo provides many advantages for large-scale expression screening, including striking similarities to human, low expense and ready availability. The goal of this proposal is to create an in situ hybridization expression resource for chicken embryos. The objectives are as follows: 1) to expand an existing gene expression screening effort, called GEISHA (gallus est in situ hybridization analysis), using automated high throughput whole mount in situ hybridization analysis to map the location of expressed sequences in chick embryos through four days of development; 2) to acquire additional expression data through parallel coordinated screening efforts, from individual researchers, and from published results; 3) to house expression information in a relational database and present expression data to the research community using a freely accessible web interface. A chicken anatomical ontology will be created to describe expression patterns and to enable spatio-temporal querying, and data will be used to annotate the chicken genome. This centralized repository for in situ hybridization gene expression information will provide a valuable and comprehensive resource for the biomedical research community.