Analytical Core

分析核心将为研究人员提供各种专业分析服务， 协助开展分子、细胞和体内研究。的 分析核心将是索尔克研究所各单位之间的合作努力， 博士罗纳德埃文斯，以及一个位于加州大学圣地亚哥分校的约瑟夫博士 维茨顿分析核心的总体目标是利用专门资源， 专门知识，为调查人员提供选定的核心服务，以协助研究使命 每个项目。该核心的目标是： 具体目标1：提供高通量定量PCR分析（HT-QPCR）， 靶基因，如核受体或影响炎症的靶基因， 动脉粥样硬化该平台将允许快速探索各种影响机制， 干扰巨噬细胞和B-1细胞中的核激素受体表达， 其他细胞或组织。 具体目标2：提供细胞因子和细胞外基质的高通量和高灵敏度分析， 通过使用Bioplex，在小样本体积（如血浆或培养液）中检测趋化因子 悬浮阵列蛋白质组分析。 具体目标3：提供免疫服务，包括产生抗原特异性 抗血清和单克隆抗体，试剂的制备和各种性能 免疫学测定，例如，所研究的鼠模型的血浆中的抗体水平。 具体目标4：提供脂质和脂蛋白的定量和定性分析， 所需的分离的脂蛋白组分，如LDL，以及提供标准化的修饰的 脂蛋白，如氧化LDL和mmLDL，供所有研究者使用。

The Analytical Core will provide a variety of specialized analytical services for the investigators of the Program Project to assist in the conduct of the molecular, cellular and in vivo studies. The Analytical Core will be a collaborative effort between units at the Salk Institute under the direction of Dr. Ronald Evans, and one located at the University of California, San Diego under Dr. Joseph Witztum. The overall goal of the Analytical Core is to take advantage of specialized resources and expertise to provide investigators with selected core services that will assist in the research mission of each Project. The Aims of this Core are: Specific Aim 1: To provide high throughput quantitative PCR analysis (HT-QPCR) of selected target genes, such as nuclear receptors or target genes that impact inflammation and atherosclerosis. This platform will allow rapid exploratory mechanism of impact of various perturbations on nuclear hormone receptor expression in macrophages and B-1 cells, as well as in other cells or tissue. Specific Aim 2: To provide high throughput and highly sensitive analysis of cytokines and chemokines in small sample volumes, such as plasma or culture fluids, through use of Bioplex suspension array proteomic profiling. Specific Aim 3: To provide immunological services including the generation of antigen-specific antisera and monoclonal antibodies, preparation of reagents and performance of a variety of immunological assays, as for example, antibody levels in plasma of murine models under study. Specific Aim 4: To provide quantitative and qualitative analysis of lipids and lipoproteins, to prepare isolated lipoprotein fractions needed, such as LDL, as well as provision of standardized modified lipoproteins, such as oxidized LDL and mmLDL for use by all investigators.