Biospecimen Extraction

生物菌素提取资源(BER)是一个标准化、高质量、 价格合理的从各种来源提取的DMA和RNA。BER执行基于聚合酶链式反应的分析 这些核酸包括焦磷酸测序用于单核苷酸多态的基因分型和 CpG岛甲基化检测。此外，BER还与非实验室调查人员进行磋商，以 帮助他们实现与该设施相关的实验室目标。误码率位于新建造的 基础科学研究大楼(BSRB)。误码率使用最先进的仪器 包括Autopure LS自动DMA萃取机和PSQ焦化测序仪。误码率已经 一个由8人组成的监督委员会。网站提供有关服务的信息、准备指南 用于DMA提取的样品和由该设施执行的其他程序，以及应 请求服务时已完成。在目前的赠款期间，35个不同的组织使用了误码率 来自17个项目的调查人员，98%的使用率来自拥有同行评审资金的调查人员。 大多数DMA提取来自人类外周血白细胞或唾液。涨幅超过 与2002年的采掘量相比，过去一年的采掘量增加了400%。该基金的资金来源 BER在33年(2008年7月1日至2009年6月30日)的拟议业务预算为14%，来自德克萨斯发电公司的53% 来自用户费用，34%来自CCSG。未来的计划包括继续扩大到大型项目，如 作为“LBJ患者病史数据库--血清/DNA库”和“组织和血液储存库” 在癌症预防中心的病人身上。

The Biospecimen Extraction Resource (BER) is a centralized laboratory for standardized, high-quality, reasonably-priced DMA and RNA extraction from various sources. The BER performs PCR-based analysis of these nucleic acids including pyrosequencing for genotyping of single nucleotide polymorphisms and for detection of CpG island methylation. In addition, the BER consults with non-laboratory-based investigators to help them accomplish their laboratory-based goals related to the facility. The BER is located in the newlyconstructed Basic Science Research Building (BSRB). The BER uses state-of-the-art instrumentation including the Autopure LS automated DMA extraction machine and the PSQ pyrosequencer. The BER has an 8-member oversight committee. A website provides information about services, guidelines for preparing samples for DMA extraction, and other procedures performed by the facility, as well as forms to be completed when requesting service. In the current grant period, the BER was used by 35 different investigators from 17 programs with 98% of usage coming from investigators having peer-reviewed funding. Most of the DMA extractions are from human peripheral blood leukocytes or saliva. An increase of over 400% has been seen over the past year compared to those extracted in 2002. The source of funds for the proposed operating budget of the BER for year 33 (July 1, 2008-June 30, 2009) is 14% from TexGen, 53% from user fees, and 34% from the CCSG. Future plans include continued expansion to large projects such as TexGen, the "LBJ Patient History Database-Serum/DNA Bank" and the "Repository of Tissue and Blood on Patients in the Cancer Prevention Center."