Streamlined Template Preparation for Advanced Sequencing Methods

描述(申请人提供)：下一代测序技术的出现使研究人员能够进行研究并发现以前在经济上或技术上不可行的东西。例如，对肿瘤组织内基因组序列的精确了解可以作为治疗的指标(类似于乳腺癌的erbB-2受体基因)。尽管许多研究和开发工作都投入到了实际产生序列数据的核心化学和仪器上，但很少有新技术被应用于高通量样品的制备或临床使用。特别是，使用珠子作为扩增的坚实支撑并帮助在测序芯片上定位样本的测序系统仍然需要繁琐的手工处理。随着实验室扩大规模，使用新的测序技术，他们很快就意识到操作中的瓶颈是样品准备。在这个第二阶段的应用中，我们建议采取远远超出最初第一阶段文库建设范围的样品制备步骤。我们建议自动化整个样品制备过程，从DNA片断之后开始，通过构建文库，直到连接到芯片之前结束。拟议的系统将与一些开始进入市场的下一代系统兼容。

DESCRIPTION (provided by applicant): The advent of next-generation sequencing technologies is allowing researchers to perform studies and make discoveries which previously were not economically or technically feasible. For example, precise knowledge of the genomic sequence within neoplastic tissues can be an indicator for treatment (similar to the erbB-2 receptor gene, Her-2, for breast cancer). Although much research and development effort has gone into the core chemistries and instruments which actually produce sequence data, few new techniques have been applied to the preparation of samples for high-throughput or use in the clinic. In particular, sequencing systems that use beads as both the solid support for amplification and to help localize samples on a sequencing chip still require tedious processing by hand. As labs scale up to use the new sequencing technologies, they are very quickly realizing that the bottleneck in the operation is in the sample preparation. In this Phase II application, we propose to take the sample preparation steps far beyond the original Phase I scope of library construction. We propose to automate the entire sample preparation process, starting just after DNA fragmentation, through library construction and ending just prior to attachment to a chip. The proposed system will be compatible with a number of next generation systems which are starting to enter the market.