In-Vivo, Wireless Fast-Scan Cyclic Voltammetry System

描述(申请人提供)：顶峰技术和堪萨斯大学的George Wilson和Michael Johnson教授开发并成功测试了一种原型设备，可以在自由活动的大鼠身上无线收集、分析和传输快速扫描循环伏安(FSCV)数据。FSCV原型系统与直径为10 5米的微电极兼容。该系统可以测量生理上相关的细胞外多巴胺水平。在这一第二阶段的工作中，将开发、测试和验证多巴胺的完整交钥匙无线系统。还将测试检测其他单胺类神经递质的能力，如去甲肾上腺素和5-羟色胺。这项工作将包括开发带有集成连接器(直径5-50 5米)的电极、集成刺激器、安装微型驱动器、优化检测极限、尽可能扩展到多个电极，以及完成商业上可行的采集和分析软件包。分析包将包括与外部刺激、视频或其他相关事件的同步。该系统将在内部进行验证，并由独立实验室进行Beta测试。与目前最先进的产品相比，拟议产品的开发是一个非常重要的进步。它将使研究人员能够测量自发的亚秒级神经递质释放事件，同时在自由活动的动物中进行详细的行为研究。对非拴系动物的行为研究提供了正常行为和功能神经化学之间联系的关键知识，无线快速循环伏安系统将是研究人员在行为、记忆、学习、成瘾机制和睡眠方面的主要资产。与公共健康相关：受异常多巴胺信号严重影响的两种疾病是精神分裂症和帕金森氏症。据估计，美国有290万人患有精神分裂症。大约有150万美国人患有帕金森氏症。根据2000年的估计，每年因精神分裂症和帕金森氏症而造成的直接医疗成本为700亿美元。

DESCRIPTION (provided by applicant): Pinnacle Technology and Professors George Wilson and Michael Johnson of the University of Kansas, have developed, and successfully tested, a prototype device that can collect, analyze and transmit fast-scan cyclic voltammetry (FSCV) data wirelessly in freely moving rats. The prototype FSCV system is compatible with microelectrodes (<10 5m diameter). The system can measure physiologically relevant levels of extracellular dopamine. During this Phase II effort, a complete turnkey, wireless system will be developed, tested, and validated for dopamine. The ability to detect other monoamine neurotransmitters such as norepinephrine, and serotonin will also be tested. This work will include the development of electrodes with integrated connectors (5-50 5m diameter), integrated stimulus, incorporation of a microdrive, optimization of the limit of detection, expansion to multiple electrodes if possible, and completion of a commercially viable acquisition and analysis software package. The analysis package will include synchronization with external stimuli, video or other related events. The system will be validated internally and beta tested by independent laboratories. Development of the proposed product is a highly significant advancement over the current state-of-the art. It will empower researchers to measure spontaneous, sub-second neurotransmitter release events while conducting detailed behavioral studies in freely moving animals. Behavioral studies in untethered animals provide critical knowledge of the link between normal behavior and functional neurochemistry, and a wireless fast cyclic voltammetry system will be a major asset for researchers in behavior, memory, learning, addiction mechanisms and sleep. PUBLIC HEALTH RELEVANCE: Two conditions heavily influenced by abnormal dopamine signaling are schizophrenia and Parkinson's disease. Schizophrenia affects an estimated 2.9 million Americans. Approximately 1.5 million Americans suffer from Parkinson's. Based on year 2000 estimates, the yearly direct health costs due to Schizophrenia and Parkinson's is 70 billion dollars.