PATIENT SAMPLE REPOSITORY AND SPECIALIZED FLOW CYTOMETRY

此共享资源支持2个功能：患者样本库和专用流式细胞术。的 核心的储存功能包括获取适当的患者样本、隔离和 单核细胞的冷冻保存，血浆或血清的分离和储存，数据库的维护 注释患者样本并将样本分发给项目研究者。我们将保管 通过确保所有样本都被去识别化并被分配编码的唯一标识， 号码我们还将验证是否符合我们的内部监管政策，该政策规定， 仅可从已签署IRB批准的知情同意书的患者中获得并进行研究 文档.核心的流式细胞仪功能包括多参数分析异质性 样品和高速细胞分选以获得高度纯化的限定祖细胞群体的群体。 该核心的具体目标如下： 1.确保维护患者隐私，并验证是否已获得知情同意， 所有样品的采集和研究。 2.确保采集临床研究方案中指定的适当样本 节目调查员。 3.从患者样本中分离并冷冻保存单核细胞和血浆或血清成分 供项目研究人员进行后续研究。 4.维护一个包含病理、细胞遗传学和分子信息的注释数据库， 从骨髓性白血病患者获得的临床样品。 5.确保样本以及时和公平的方式分发给实验室研究者， 本申请中提出的实验。 6.提供专业化的多参数分析和高速细胞分选，获得高纯度的 用于功能和遗传分析的群体

This shared resource supports 2 functions: patient sample repository and specialized flow cytometry. The epository functions of the core include the acquisition of appropriate patient samples, isolation and cryopreservation of mononuclear cells, separation and storage of plasma or serum, maintenance of a database annotating patient samples and distribution of samples to program investigators. We will safeguard patient privacy by ensuring that all samples are de-identified and are assigned coded unique identifying numbers. We will also verify compliance with our internal regulatory policies which stipulate that samples may be obtained and studied only from patients who have signed an IRB approved informed consent document. The flow cytometry functions of the core include multiparameter analysis of heterogeneous samples and high-speed cell sorting to obtain highly purified populations of defined progenitor populations. The specific aims of this core are listed below: 1. To ensure the maintenance of patient privacy and to verify that informed consent has been obtained for acquisition and study of all samples. 2. To ensure the acquisition of appropriate samples as designated in clinical research protocols carried out by program investigators. 3. To isolate and cryopreserve mononuclear cells and plasma or serum components from patient samples for subsequent study by program investigators. 4. To maintain an annotated database containing pathologic, cytogenetic and molecular information for clinical samples obtained from patients with myeloid leukemias. 5. To ensure that samples are distributed in a timely and equitable fashion to laboratory investigators for experiments proposed in this application. 6. To provide specialized multi-parameter analysis and high-speed cell sorting to obtain highly purified populations for functional and genetic analysis