Research Methods Core

该核心的目的是提供一套通用的最先进的生化和 对CIDAR研究人员的遗传分析与检验与以下各项相关的假说 抗抑郁药物反应的预测。核心实验室将提供以下评估 下丘脑-垂体-肾上腺(HPA)轴功能、白细胞介素6和C-反应蛋白(C-RP)； 抗抑郁药物浓度和详细的基因分析。后者包括罚款 候选基因的定位，选择“标记SNPs”以及基因数据管理。 将特别强调为每个人开发密集的多态图谱 候选基因用于鉴定不同种族群体间的连锁不平衡程度。这些 将向统计建模核心提供数据。使这些确定由 使用一套通用的高质量分析模式的中央设施将在 所有受试者都参加了这项为期5年的研究。

The purpose of this Core is to provide a common set of state-of-the-art biochemical and genetic analyses to CIDAR researchers relevant to testing various hypotheses related to prediction of antidepressant response. The laboratories of the Core will provide assessments of hypothalamic-pituitary-adrenal (HPA) axis function, interleukin-6 and C-reactive protein (C-RP), antidepressant drug concentrations, and detailed genetic analyses. The latter includes fine mapping of candidate genes, choosing "tagging SNPs" as well as genetic data management. Particular emphasis will be placed on development of a dense polymorphism map for each candidate gene to identify the degree of linkage disequilibrium in different ethnic groups. These data will be provided to the statistical modeling core. Having these determinations performed by a central facility using a common set of high quality assay modalities will provide consistency across all of the subjects enrolled in this 5 year study.