Gene Targeting Core Facility

基因打靶核心设施(GTCF)是一个完全建立起来的核心资源，可以为以下方面提供专家服务 胚胎干细胞基因打靶用于小鼠模型的发展。主要服务 这个核心所产生的是将研究人员的基因打靶载体和生产的ES细胞系 可以发育成嵌合小鼠，通过它们的生殖系传递适当的基因组修改(S)。 利用这个核心的研究人员已经成功地培育出了各种基因敲除和小鼠 疾病的模型。GTCF是UAB转基因小鼠设施的组成部分 我们不仅通过GTCF为基因打靶提供专家服务，还为囊胚提供专家服务 显微注射靶向ES细胞克隆或DNA构建物以产生转基因小鼠模型， 以及与小鼠研究有关的一系列服务(例如胚胎的冷冻保存，再衍生， 等)。转基因动物设施与UAB的其他核心资源具有战略联系，包括 临床营养研究中心小动物表型分析核心， 表型评估研究动物病理实验室和专门的老鼠繁育核心 (基因工程小鼠和标记辅助遗传学核心)总体而言，我们拥有主要的资源来 为中心的研究人员制作和鉴定小鼠模型。

The Gene Targeting Core Facility (GTCF) is a fully established core resource to provide expert services for gene targeting of embryonic stem (ES) cells for the development of mouse models. The primary service orovided by this core is that of taking an investigator's gene targeting vector and producing ES cell lines that can be developed into chimeric mice that transmit the pertinent genomic modification(s) through their germline. Investigators utilizing this core have successfully produced a variety of gene knockouts and mouse models of disease. The GTCF is an integral component of the UAB Transgenic Mouse Facility that collectively provides expert services for not only gene targeting via the GTCF, but also blastocyst micro-injection of targeted ES cell clones or DNA constructs for the production of transgenic mouse models, as well as a host of services related to research with mice (e.g. cryopreservation of embryos, rederivation, etc.). The Transgenic Animal Facility is strategically linked with other core resources at UAB including the CCC Small Animal Imaging Core, Clinical Nutrition Research Center Small Animal Phenotyping Core, Research Animal Pathology Laboratory for phenotype assessment, and a specialized mouse-breeding core (Genetically Engineered Mouse and Marker-Assisted Genetics Core). Overall, we have premier resources to make and characterize mouse models for the Center investigators.