PATHOLOGY SUPPORT FOR NTP

建立受试者合同是为了提供广泛的常规和专业病理学服务，以支持国家毒理学计划（NTP）根据合同开展的研究，并对这些研究生成的病理学标本进行补充研究。该合同还为NIEHS内部研究人员进行的研究和其他NIEHS合同研究提供关键病理学（包括分子病理学）支持。具体而言，本合同支持以下服务：1）尸检和内部尸检协助; 2）组织学（组织修剪和处理、载玻片制备和染色）; 3）组织病理学评价/解释; 4）应用专门的定性和定量形态学程序（免疫组织化学、形态计量学、细胞增殖、凋亡）; 5）适应，发展，完善，应用细胞和分子生物学新技术，包括（但不限于）基因分型、DNA测序、原位杂交、DNA和RNA分离和扩增、以及真实的实时定量和原位PCR，以及这些技术应用的培训; 6）病理学同行评议（包括病理学工作组）和病理学评价的质量评估以及对这些活动的技术支持; 7）病理学数据输入;和8）病理学数据或技术的介绍或出版，或对这些活动的支持。过去一年完成了关于这一合同的下列工作：为国家毒理学方案完成了9项病理学和病理学工作组审查;为19个内部项目和国家毒理学方案项目制备了组织学载玻片，其中一些项目还包括动物尸检;为2个内部项目进行了专门的病理学评价;为国家毒理学和毒理学研究所组织学和免疫组织化学实验室提供了现场技术支持; 2个项目与准备病理学数据以供介绍或出版有关。此外，还有许多其他项目正在进行中的NTP和NIEHS，其中一些是涉及长期圈养动物的长期项目。

The subject contract was established to provide a broad range of routine and specialized pathology services in support of studies conducted under contract by the National Toxicology Program (NTP) and for supplemental studies on pathology specimens generated through these studies. The contract also provides critical pathology (including molecular pathology) support for studies conducted by in-house investigators at NIEHS and other NIEHS contract studies. Specifically, the following services are supported by this contract: 1) necropsy and in-house necropsy assistance; 2) histology (tissue trimming and processing, slide preparation, and staining); 3) histopathological evaluation/interpretation; 4) application of specialized qualitative and quantitative morphological procedures (immunohistochemistry, morphometrics, cell proliferation, apoptosis); 5) adaptation, development, refinement, application of new techniques in cellular and molecular biology including (but not limited to) genotyping, DNA sequencing, in situ hybridization, DNA and RNA isolation and amplification, and real time, quantitative, and in situ PCR, as well as training in the application of these techniques; 6) Pathology Peer Review (including Pathology Working Groups) and Quality Assessment of pathology evaluations and technical support for these activities; 7) pathology data entry; and 8) presentation or publication of pathology data or techniques, or support for these activities. The following work was completed on this contract in the past year: 9 pathology and pathology working group reviews have been completed for the National Toxicology Program; preparation of histologic slides for 19 in-house and NTP projects some of which also included animal necropsies; specialized pathology evaluations for 2 in-house projects; on-site technical support in the NIEHS Histology and Immnohistochemistry Laboratories; and 2 projects related to preparation of pathology data for presentation or publication. In addition, there are many other projects for the NTP and NIEHS that are in progress, some of which are long term projects involving housing of animals for long periods.