National Heart, Lung, and Blood Institute Proteomics Initiative

这个蛋白质组学中心聚集了一个高度跨学科的团队，开发创新的平台技术，分析与全身疾病过程相关的细胞内和分泌的血细胞蛋白。该中心建议分析自身免疫性疾病的血液成分，特别是系统性红斑狼疮(SLE)和类风湿性关节炎(RA)。三个项目将各自开发和应用新的蛋白质组学技术，以研究小鼠和人类SLE和RA血液成分的异常。此外，还将开发新的分析算法，以整合和分析相关但不同的蛋白质组、基因组和临床数据大集。这一提议的一个优点是现有技术在相关生物学问题上的新应用，包括用于同时测量多种激酶状态的多色流式细胞术、基于微流控和毛细管电泳法的细胞因子和自身抗体的蛋白质组学分析，以及用于分析自身抗体反应的平面蛋白质阵列。与生物应用紧密结合的技术和方法开发是该中心的优势所在。系统地获取与结缔组织疾病相关的大量数据是一种有价值的新方法，它可能导致诊断和治疗的新进展。

This Proteomics Center brings together a highly interdisciplinary team to develop innovative platform technologies that analyze intracellular and secreted blood cell proteins related to systemic disease processes. This Center proposes analysis of the blood components in autoimmune disease, specifically focusing on systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Three projects will each develop and apply novel proteomics technologies to study aberrances in the blood components in murine and human SLE and RA. In addition, novel analysis algorithms to integrate and analyze related but disparate megasets of proteomic, genomic, and clinical data will be developed. A strength of this proposal is the novel applications of existing technologies to relevant biological questions, including polychromatic flow cytometry for simultaneous measurement of multiple kinase states, microfluidic and capillary electrophoresis-based proteomic analysis of cytokines and autoantibodies, and planar protein arrays for analysis of autoantibody responses. Technology and method development tightly coupled to biological applications is a strength of this Center. The systematic acquisition of large amounts of data related to connective tissue diseases is a valuable new approach which may lead to new advances in diagnosis and treatment.