National Heart, Lung, and Blood Institute Proteomics Initiative

Kenneth威廉姆斯博士NHLBI-HL-02-04 耶鲁大学 我们建议定义蛋白质和磷蛋白表达的相对水平的变化，其基础，定义，并导致相关的病理生理学：造血，血管生物学和高血压。为了帮助分析将产生的大量蛋白质表达数据，并将这些数据存档，以便通过Web界面进行分析，查询和传播，我们将设计和建立NHLBI/耶鲁表达数据库。该数据库将与耶鲁微阵列数据库中的mRNA表达数据以及功能和其他生物信息学数据库链接和交叉相关。为了充分利用蛋白质谱分析的能力来揭示在感兴趣的病理生理学中起关键作用的蛋白质，将需要开发同样有效的生物技术来调节这些蛋白质靶标的体内活性。因此，我们还提出了一个紧密协调的计划，用于改善ND，利用细胞可渗透的合成肽递送系统来阻断特定的蛋白质：蛋白质相互作用-目的是增加我们对疾病过程的理解，这反过来又会导致对这些疾病更有效的治疗。

Dr. Kenneth Williams NHLBI-HL-02-04 Yale University We propose to define changes in the relative levels of protein and phosphoprotein expression that underlie, define, and result from pathophysiologies related to: hematopoiesis, vascular biology, and hypertension. To assist with analyzing the enormous amounts of protein expression data that will be produced and to archive this data so it can be analyzed, queried, and disseminated via web interface, we will design and institute the NHLBI/Yale Expression Database. This database will be linked and cross-correlated with mRNA expression data in the Yale Microarray Database and with functional and other bioinformatics databases. To take full advantage of the ability of protein profiling to uncover proteins that play key roles in pathophysiologies of interest will require that equally effective biotechnologies be developed for modulating the in vivo activities of these protein targets. Hence, we propose also a tightly coordinated program for improving nd utilizing cell permeable synthetic peptide delivery systems to block specific protein:protein interactions - with the goal of increasing our understanding of the disease process which, in turn, should lead to more effective treatments for these diseases