Animal Production Core

动物生产核心（APC）将继续提供选择性饲养的P/NP（酒精偏好和- nonprefering）和HAD 1 -2/LAD 1 -2（重复高和低酒精饮用）大鼠进行研究。为此 竞争性更新，APC还将提供用于研究目的的选择性繁殖的复制HAP/HAP (high和低酒精偏好）和cHAP（由HAP 1 × HAP 2杂交产生的杂交HAP）小鼠。这些 啮齿动物将提供给由我们的印第安纳州ARC支持或附属于我们的研究人员， 其他由NIAAA资助的校内PI，包括R 01，U 01，F31和我们的T32机构培训 格兰特. P和HAD 1 -2大鼠是人类疾病酒精中毒的良好动物模型。P/NP 大鼠，并在较小程度上，啮齿动物的HAD/LAD和HAP/LAD复制系已被广泛 从行为学、神经化学、神经药理学和QTL角度表征。P大鼠 也构成了一个很好的模型来研究“狂欢”饮酒，酒精剥夺效应（ADE， 相当于“复发”饮酒或“渴望”）和青少年酗酒。此外，P大鼠是一种 这是一个很好的动物模型，可以更好地定义乙醇奖励作用的神经回路和 CREB（cAMP反应元件结合）和神经肽Y基因表达与抗焦虑作用的关系 酒精具体目标是：1）继续保持我们的核心群体的选择性育种P/NP 大鼠2)为校园研究人员生产足够数量的选择性繁殖的P/NP和HAD 1- 2/LAD 1 -2大鼠。3)继续选择性繁殖复制HAP/cHAP小鼠品系和cHAP小鼠 线，并产生足够的动物在校园内的研究。4)为了维持N/Nih大鼠的群体（ 用于HAD/LAD和HAS/LAS复制系的选择性育种的基础原种），因为这 NIH没有保存异质性储备液，它是比较HAD 1 -2的研究中的相关对照线。 线与其对比的LAD 1 -2线。5)继续协助建立互惠的同类系 从近交系P/NP菌株，以进一步缩小关键区域的Chr 4的酒精偏好在 LOD评分为9.2的QTL。APC和我们的IARC的成功将在以下方面取得重要进展： 了解酒精中毒的遗传决定因素和神经回路。这些科学进步 这将为药物开发铺平道路，以治疗酒精中毒的各个方面。

The Animal Production Core (APC) will continue to supply selectively bred P/NP (alcohol-preferring and - nonpreferring) and HAD1-2/LAD1-2 (replicate high and low alcohol drinking) rats for research. For this competing renewal, the APC will also supply for the purpose of research selectively bred replicate HAP/LAP (high and low alcohol-preferring) and cHAP (crossed HAP produced by HAP1 x HAP2 cross) mice. These rodents will be provided to on-campus investigators supported by or affiliated with our Indiana ARC and other on-campus Pis who are funded by NIAAA with R01s, U01s, F31s, and our T32 Institutional Training Grant. The P and HAD1-2 rats are excellent animal models of the human disorder, alcoholism. The P/NP rats, and to a lesser degree, the HAD/LAD and HAP/LAP replicate lines of rodents have been extensively characterized from behavioral, neurochemical, neuropharmacological, and QTL perspectives. The P rats also constitute an excellent model to study "binge" drinking, the alcohol-deprivation effect (ADE, the equivalent of "relapse" drinking or "craving") and adolescent alcohol abuse. Furthermore, the P rat is an excellent animal model to better define the neurocircuitry of the reward action of ethanol and the relationship of CREB (cAMP-responsive-element binding) and neuropeptide Y gene expression to the anxiolytic effect of alcohol. The Specific Aims are: 1) To continue to maintain our nucleus colonies of selectively bred P/NP rats. 2) To produce for on-campus researchers sufficient number of selectively bred P/NP and HAD1- 2/LAD1-2 rats. 3) To continue to selectively breed the replicate HAP/LAP mouse lines and the cHAP mouse line and to produce sufficient animals for on-campus research. 4) To maintain a colony of N/Nih rats (the foundation stock for the selective breeding of the HAD/LAD and HAS/LAS replicate lines) because this heterogeneous stock is not kept at NIH and it is a relevant control line in studies that compare the HAD1-2 lines with their contrasting LAD1-2 lines. 5) To continue to assist in the creation of reciprocal congenic lines from inbred P/NP strains in order to further narrow the critical region of Chr 4 for alcohol preference in the QTL with a LOD score of 9.2. The success of the APC and our IARC will provide important advances in the understanding of the genetic determinants and neurocircuitry of alcoholism. These scientific advances in turn will pave the way for medication development to treat various aspects of alcoholism.