CORRELATION OF MATRIX METALLOPROTEINASE AND FUNCTIONAL OUTCOME FOLLOWING TRAUMA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 创伤性脑损伤使基质金属蛋白酶-2和-9水平升高，这些水平的升高与格拉斯哥预后评分(GOS)和兰乔·洛斯·阿米戈斯认知功能量表(LCFS)相关。 具体目的：通过将导管插入脑室，对严重脑损伤患者进行脑脊液(CSF)压力监测。压力被监测，当压力升高时，脑脊液被取出。脑脊液反映了脑间质液体的变化，对脑脊液中底物的测量提供了大脑中正在发生的生化变化的指示。包括MMPs在内的脑脊液蛋白的增加在几种神经系统疾病中都会发生。我们计划用一种灵敏的酶谱方法检测脑脊液中MMPs的水平。 目的：选择合适的脑创伤患者入院时、伤后24小时、72小时、脑室造口术时脑脊液标本，分析其血浆和脑脊液中基质金属蛋白酶水平。 目的：建立脑脊液和血浆中基质金属蛋白酶-2和基质金属蛋白酶-9表达和活化的时程。 目的3：探讨GOS和LCFs与基质金属蛋白酶水平的相关性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Traumatic brain injury (TBI) increases Matrix Metalloproteinase (MMP)-2 and -9 and the increase in these levels correlate with the Glasgow Outcome Score (GOS) and Rancho Los Amigos Cognitive Functioning Scale (LCFS). Specific aims: Patients with severe brain injury undergo monitoring of the cerebrospinal fluid (CSF) pressure by the insertion of a catheter into the cerebral ventricles. The pressure is monitored and the CSF is withdrawn when the pressure is elevated. CSF reflects the changes in the brain interstitial fluid and measurements of substrates in the CSF provide an indication of the biochemical changes that are occurring in the brain. An increase in CSF proteins, including MMPs, occurs in several neurological diseases. We plan to measure the levels of MMPs in the CSF by a sensitive zymographic method. Aim 1: To carefully select an appropriate TBI cohort for MMP level analysis in plasma and CSF and to obtain samples of CSF from brain injured patients at the time of admission, 24 and 72 hours after the injury, and at the time of the removal of the ventriculostomy. Aim 2: To establish the time course of MMP-2 and -9 expression and activation in CSF and plasma. Aim 3: To determine the correlation of GOS and LCFS to MMP levels.