Rodent Cell Line Authentication

描述(申请人提供)：这项研究的长期目标是开发一种有效的方法来鉴定啮齿动物细胞系。细胞系的错误识别以及种间和种内的交叉污染普遍存在，并对生物医学研究产生了重大的负面影响。短串联重复序列(STR)分析已被证明是鉴定人类细胞系的一种有效手段，已为大量人类细胞系建立了STR基因图谱，其中大部分数据可在ATCC STR图谱数据库中公开访问。目前尚不存在用于鉴定啮齿动物细胞系的同等资源。为了填补这一空白，这项应用的具体目标是开发一种基于多重微卫星标记的测试，以生成小鼠和大鼠细胞系的独特遗传图谱，并建立这些图谱的数据库，用于啮齿动物细胞系的鉴定。通过开发这些资源，将有可能为研究界提供一种经济有效和准确的啮齿动物细胞系鉴定方法。公共卫生相关性：细胞系的使用是基础生物医学研究的基石，确保细胞系的真实性的能力对于确保指导影响公共健康的科学进步的研究结果的完整性和有效性至关重要。建立一种为啮齿动物细胞系生成独特基因图谱的方法为验证研究实验室中使用的这些细胞系的真实性提供了一种快速、灵敏和准确的手段。

DESCRIPTION (provided by applicant): The long term goal of this research is to develop an efficient method to authenticate rodent cell lines. Misidentification as well as inter- and intra-species cross contamination of cell lines is widespread and has a substantial negative impact on biomedical research. The use of short tandem repeat (STR) analysis has been shown to be an effective means of authenticating human cell lines, STR genetic profiles have been established for a large number of human cell lines, and much of this data is publicly accessible in the ATCC STR Profile Database. Equivalent resources do not currently exist for authentication of rodent cell lines. In order to fill this gap, the specific aims of this application are to develop a multiplex microsatellite marker-based test to generate unique genetic profiles for mouse and rat cell lines and to build a database of these profiles to use for rodent cell line authentication. By developing these resources, it will be possible to provide a cost-effective and accurate method for rodent cell line authentication for the research community. PUBLIC HEALTH RELEVANCE: The use of cell lines is a cornerstone of basic biomedical research and the ability to ensure the authenticity of the cell lines is critical to ensuring the integrity and validity of the research results that guide scientific advances affecting public health. Establishing a method for generating unique genetic profiles for rodent cell lines provides a quick, sensitive, and accurate means to verify the authenticity of those cell lines being used in research laboratories.