Reagents to Enhance Detection of Raw and Biologically Transformed Nanomaterials

越来越多的工程纳米材料在工业和消费应用中的使用已经 对他们的环境健康安全提出了严重关切(EH和S)。一个特别值得关注的问题是 对于职业暴露于高度分散的球形和纤维形式的原材料- 就像形状一样。研究人员试图量化纳米材料与生物系统的相互作用 建立纳米EH和S风险的预测模型。然而，由 所采用的模型系统、缺乏NP标准、研究人员未能量化或 报告所调查的NP的物理化学性质，以及供应不足 灵敏的分析仪器，共同阻碍了在实现这些方面的进展 呃&S进球了。在拟议的项目中，我们试图克服检测纳米颗粒方面的限制 通过开发一组试剂(NProbe)来增强它们在生物组织中的存在 由于它们的大小，可能会被其他方法遮挡住。NProbe试剂将 其功能与用于检测和定量标准蛋白质的功能相似 免疫组织化学分析。对原始NP和转化后的NP具有高结合亲和力的NProbe 纳米材料将使用基于蛋白质的(抗体和纤维连接蛋白)噬菌体展示来产生 技术感兴趣的纳米材料包括碳纳米管、金属氧化物、金属和 半导体公司。我们将验证NProbe与原始纳米材料的结合，并测试其 对相关和不同成分的样品的交叉反应性。N探测绑定到 还将展示通过穿透皮肤而转化的纳米材料。我们 相信nProbe试剂将对研究人员评估纳米颗粒组织有用 所有哺乳动物、脊椎动物、无脊椎动物系统中的相互作用以及对 环境中的纳米颗粒。NProbe试剂的成功开发将产生 纳米颗粒风险评估研究的变革性效应。

The increasing use of engineered nanomaterials in industrial and consumer applications has raised serious concerns about their environment health safety (EH&S). A particular concern is for occupational exposure to raw materials in the form of highly dispersed spherical and fiber- like shapes. Researchers seek to quantify nanomaterial interactions with biological systems and to develop predictive models of nanoparticle EH&S risk. However, limitations imposed by the model systems employed, the lack of NP standards, the failure of researchers to quantify or report the physiochemical properties of the NP investigated, and the inadequate availability of sensitive analytical instrumentation, have collectively hampered progress in attaining these EH&S goals. In the proposed project we seek to overcome limitations in detecting nanoparticles by developing a set of reagents (NProbes) that will enhance their presence in biological tissues that due to their size may be obscured from detection by other means. NProbe reagents will function in a similar fashion to those used to detect and quantify proteins in standard immunohistological analysis. NProbes with high binding affinity to both raw NP and transformed nanomaterials will be generated using protein-based (antibody and fibronectin) phage display technology. Nanomaterials of interest include carbon nanotubes, metal oxides, metals, and semiconductors. We will validate the binding of NProbes to raw nanomaterials and test their cross reactivity to samples of related and dissimilar composition. NProbe binding to nanomaterials transformed by penetration through skin will be also be demonstrated. We believe that NProbe reagents will be useful to researchers assessing nanoparticles tissue interactions in all mammalian, vertebrate, invertebrates systems as well as detection of nanoparticles in the environment. Successfully development of NProbe reagents will have a transformative effect on nanoparticle risk assessment research.