A NOVEL HIGH-CAPACITY ION TRAP-QUADRUPOLE TANDEM MASS SPECTROMETER

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们最近开发了一种新型的高容量离子阱-四极杆质谱仪，我们之前已经描述过： A.N. Krutchinsky，H.科恩，B.T.一种新型高容量离子阱-四极杆串联质谱仪Int J Mass Spectrom，268（2007）93-105 我们已经获得了美国专利 我们通过构建第二代仪器继续这一开发，如下所述： 开发了一种与飞行时间（TOF）质谱仪耦合的高容量离子阱，以对离子阱中所有存储的离子进行全面的联动扫描分析。该方法涉及一种新颖的锥形几何形状的高容量离子阱，可以存储超过10^6个离子（范围8004000 m/z）而不降低其性能。离子被存储并作为m/z的函数从高容量离子阱中扫描出来，碰撞破碎并通过TOF分析。对从离子阱喷射出的所有物质的前体和碎片都实现了精确的质量分析。我们展示了全面的连接扫描识别磷酸肽与其相应的非磷酸化肽的混合物的方法。 已经发表了一篇描述这项工作的论文： S. Myung，H.科恩，D. J.C.费尼Padovan，A.N. Krutchinsky，B.T.查特“高容量离子阱耦合到飞行时间质谱仪，用于无扫描损失的综合链接扫描”，国际质谱杂志2010年。In press

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We have recently developed a novel high capacity ion trap-quadrupole mass spectrometer, which we have described previously: A.N. Krutchinsky, H. Cohen, B.T. Chait A novel high-capacity ion trap-quadrupole tandem mass spectrometer Int J Mass Spectrom, 268 (2007) 93-105 and for which we have been granted a US patent. We have continued this development by constructing a second generation instrument as described below: A high-capacity ion trap coupled to a time-of-flight(TOF) mass spectrometer has been developed to carry out comprehensive linked scan analysis of all stored ions in the ion trap. The approach involves a novel tapered geometry high-capacity ion trap that can store more than 10^6 ions (range 8004000 m/z) without degrading its performance. Ions are stored and scanned out from the high-capacity ion trap as a function of m/z, collisionally fragmented and analyzed by TOF. Accurate mass analysis is achieved on both the precursor and fragments of all species ejected from the ion trap. We demonstrate the approach for comprehensive linked-scan identification of phosphopeptides in mixtures with their corresponding unphosphorylated peptides. A paper describing this work has been published: S. Myung, H. Cohen, D. Feny¿, J.C. Padovan, A.N. Krutchinsky, B.T. Chait. "High-capacity ion trap coupled to a time-of-flight mass spectrometer for comprehensive linked scans with no scanning losses", International Journal of Mass Spectrometry 2010. In press