IMPROVED HIGH SPEED AFFINITY ISOLATION OF PROTEIN COMPLEXES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们正在完善我们的亲和分离技术，使蛋白质复合物的纯化越来越快。我们已经证明工作良好的一种策略涉及使用小直径磁珠。我们正在计划一些进一步的实验，以帮助elicidate的隔离时间对珠直径的依赖性的理论模型。其他策略涉及使用小体积净化装置，包括小体积尺寸排阻色谱法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are refining our affinity isolation techniques to enable increasingly rapid purifications of protein complexes. One tactic that we have shown to work well involves the use of small diameter magnetic beads. We are planning some further experiments to help elicidate a theoretical model for the dependence of the isolation time on the bead diameter. Other tactics involve use of small volume cleanup apparatus, including low volume size exclusion chromatography.