USING ANNOTATED PEPTIDE MASS SPECTRUM LIBRARIES FOR PROTEIN IDENTIFICATION

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 描述了一种用于创建用相应肽序列注释的串联质谱库的系统。该系统基于全球蛋白质组机器数据库（GPMDB）中当前可用的注释谱。通过将用相同肽序列、序列修饰和母体离子电荷注释的光谱一起平均化来创建文库光谱。构建文库，以便将实验肽串联质谱与文库中的肽串联质谱进行比较，从而基于对实验光谱与文库内容物的相似性进行评分来鉴定肽序列。构建了执行这种类型的文库搜索的软件实现，并成功地用于获得序列鉴定。智人、小家鼠和酿酒酵母蛋白质组的注释串联质谱文库和搜索软件可供其他研究组下载和使用。这些和其他进展进行了讨论： 使用全球蛋白质组机器的质谱蛋白质鉴定。 放大图片作者：J. 分子生物学方法2010;673：189-202

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups. These and other advances are discussed in: Mass spectrometric protein identification using the global proteome machine. Feny¿ D, Eriksson J, Beavis R. Methods Mol Biol. 2010;673:189-202