ROS Analytical Core

核心B是ROS分析核心。这是一个新的核心，成为必要的，因为所有的项目 要求对活性氧物种(ROS)进行定量测量。测量ROS是具有挑战性的， 特别是当在体内进行时，因为细胞内ROS的多个来源，以及已知的 一种形式的ROS容易转变为另一种形式的倾向。氧化应激的一个最重要的方面， 然而，细胞中中性脂和磷脂的不饱和脂肪酸部分是否发生过氧化 细胞膜和细胞蛋白质的氧化。此外，测量特定的ROS可能无法解释 病理性疼痛的功能结局及相关发病机制。对ROS诱导的血脂的评价 蛋白质的过氧化和氧化似乎是理解潜在的 我们疼痛模型中的机制。脂质过氧化和氧化蛋白的发现将得到以下支持 实时活细胞和组织化学固定细胞成像将与Core C合作进行。 因此，ROS分析核心将测量脂质过氧化产物和氧化蛋白，以及ROS 在活细胞或固定细胞中生成。ROS分析核心由三个分支组成，分配了一个 每项工作的联合主任：1)脂质过氧化分析，2)蛋白质氧化分析，3)组织病理学 树枝。分支1)和分支2)用于化学分析，分支3)用于测量细胞内ROS 使用实时活细胞成像和来自固定细胞的组织化学成像的水平。用于使用 成像设备，该核心与核心C(成像核心)和 这一核心的组织病理学也是成像核心的联席主任。因此，ROS分析核心 成为PPG不可或缺的一部分，描述ROS在中枢和外周敏化中的作用 疼痛。

Core B is the ROS Analytical Core. This is a new core that became necessary because all the projects require quantitative measurement of reactive oxygen species (ROS). Measuring ROS is challenging, particularly when done in vivo, because of multiple intracellular sources of ROS in cells and the known propensity of one form of ROS to readily transform into another. One overriding facet of oxidative stress, however, is peroxidation of unsaturated fatty acid moieties of neutral lipids and phospholipids in cell membranes and oxidation of cellular proteins. Moreover, measuring specific ROS may not explain the functional outcomes in pathological pain and related pathogenesis. An assessment of ROS-induced lipid peroxidation and oxidation of proteins appears to be a logical choice for understanding the underlying mechanism in our pain model. The findings of lipid peroxidation and oxidation proteins will be supported by the real-time live cell and histochemical fixed cell imaging to be conducted in collaboration with Core C. Therefore, ROS Analytical Core will measure lipid peroxidation products and oxidized proteins, and ROS generation in live or fixed cells. The ROS Analytical Core is composed of three branches with an assigned Co-Director for each: 1) Lipid Peroxidation Analysis, 2) Protein Oxidation Analysis, and 3) Histopathology branches. While branches 1) and 2) are for the chemical analysis, branch 3) is to measure intracellular ROS levels using both real-time live-cell imaging and histochemical imaging from fixed cells. For the use of the imaging facility, this core works very closely with Core C (Imaging Core) and the Co-Director of Histopathology of this core is also a Co-Director of the Imaging Core. Therefore, the ROS Analytical Core becomes an integral part of the PPG in delineating the role of ROS in central and peripheral sensitization in pain.