Flow Cytometry

项目总结(见说明)； 马里兰大学马琳和斯图尔特·格林鲍姆癌症中心(UMGCC)的流式细胞术共享服务(FCS)为从事癌症生物学和其他基础科学和应用科学研究的UMGCC成员提供流式细胞仪设备和技术专长。服务对整个马里兰大学校园开放，但UMGCC约占总使用量的75%。FCS提供从功能和表型分析到细胞分类的全方位、最先进的流式细胞仪服务。分析仪器包括一台现代的全数字3激光Becton Dickinson(BD)LSR II流式细胞仪，以及两台模拟流式细胞仪：一台BD LSR 1和一台BD FACScan仪器。细胞分选使用(1)BD FACSAria I数字仪器，配备两个激光和七种颜色检测 (2)一台BD FACSVantage SE仪器，升级了BD的数字采集系统(DIVA)，配备了三台激光器，包括一台可调谐的氪激光器，可激发351 nm的紫外线。 FCS还为用户提供多个平台上的操作员辅助和自助式数据分析以及实验培训和咨询，以支持个别分析或分类实验的项目设计。 FCS的有效性从以下方面可见一斑：近年来其利用率稳步上升，使用共享服务的50多名主要研究人员，以及在共享服务帮助下进行的实验所支持的出版物和国家癌症研究所资助的项目的数量。 FCS的目标是通过扩展细胞分选能力，进一步扩大其服务的广度和范围；支持基于流式细胞仪的新型分析应用；并加强与UMGCC其他共享传感器/ICE的合作，以帮助向UMGCC调查人员介绍创新的尖端技术。

PROJECT SUMMARY (See instructions); The Flow Cytometry Shared Service (FCSS) at the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) offers flow cytometry equipment and technical expertise to UMGCC members conducting research in cancer biology and other areas of basic and applied science. Services are open to the entire University of Maryland campus, but UMGCC accounts for about 75 percent of the total use. FCSS provides full scale, state-of-the-art flow cytometry services, from functional and phenotypic analysis to cell sorting. Analytical instruments include a modern, fully digital, 3-laser Becton Dickinson (BD) LSR II flow cytometer, and two analog flow cytometers: a BD LSR 1 and a BD FACScan instrument. Cell sorting is conducted with (1) a BD FACSAria I digital instrument equipped with two lasers and seven-colordetection capability and (2) a BD FACSVantage SE instrument upgraded with BD's Digital Acquisition System (DIVA) and equipped with three lasers including a tunable krypton laser for 351 nm ultraviolet excitation. FCSS also offers users both operator-assisted and self-service data analysis on several platforms as well as experimental training and consultation to support project design for individual analytical or sorting experiments. The effectiveness of FCSS is evident from the steady increase of its utilization in recent years, the more than 50 principal investigators who are using the shared service, and the number of publications and National Cancer Institute-funded projects supported by experiments conducted with the help ofthe shared service. The goal of FCSS is to further increase the breadth and scope of its services by expanding cell-sorting capabilities; supporting novel, flow cytometry-based analytical applications; and strengthening cooperation with other UMGCC shared sen/ices to help introduce innovative, cutting-edge technologies to UMGCC investigators.