Next Generation Sequencing and Genotyping Core Facility

下一代测序和基因分型核心设施。 下一代排序组件的目标是以具有成本效益的价格向北卡罗来纳大学LCCC成员广泛提供“下一代”排序和相关服务。服务包括：人类和模型生物的全基因组鸟枪式测序、染色质IP样本测序、用数字基因表达和RNA-SEQ测量RNA表达、SNP分型和多态筛选。这个 该组件通过将复杂而昂贵的DNA/RNA测序技术放在UNC LCCC成员可以轻松获得的范围内，为癌症中心增加了价值。高度整合的基因组学和生物信息学小组与用户互动，以确保健壮的数据被适当地存档以供未来使用。这一部分是北卡罗来纳大学LCCC参与癌症基因组图谱项目和满足LCCC需求的关键。未来的计划包括增加工作人员和设备，以便可以及时提供全套下一代测序和服务，以及获得下一代技术。 DNA测序部分的目标是为北卡罗来纳大学-CH分校的研究人员使用最先进的技术快速、合理地产生高质量的测序和基因分型数据，并提供技术支持，以提高所产生的结果的价值。服务包括DNA测序和微卫星基因分型。该组件通过在以下位置生成高质量数据来为癌症中心增加价值 具有竞争力的价格，同时提供当地资源的好处，以协助：科学战略、样品制备、在困难地区进行排序，以及协助数据解释。这一组成部分拥有广泛的用户基础，2009年有220多个不同的实验室作为用户。未来的计划包括继续使用现有设备，降低化学成本，以及评估未来的测序技术选择 例如长读单分子测序技术。 高通量基因分型组件的目标是为使用最先进技术的研究人员快速、合理地产生高质量的基因分型数据。服务包括SNP基因分型、拷贝数变异和DNA甲基化图谱分析。正在探索新的技术和/或应用，以增加核心的竞争优势，并允许基因组范围协会对稀有变异进行研究。 这些核心组件由多个成员使用，在每个核心成员中，同行评审成员的使用率超过70%。拟议预算为241 797美元，不到核心业务费用的5%。预算为与成员合作采用这些新技术的关键核心工作人员提供了稳定的工资。

Next Generation Sequencing and Genotyping Core Facility. The goal of the Next Gen Sequencing component is to make "next generation" sequencing and related services widely available to UNC LCCC members at cost-effective prices. Services include: whole genome shotgun sequencing of human and model organisms, chromatin-IP sample sequencing, measuring RNA expression with both digital gene expression and RNA-seq, SNP typing, and polymorphism screens. The component adds value to the cancer center by putting complex and expensive DNA/RNA sequencing technology within easy reach of UNC LCCC members. Strongly integrated genomics and bioinformatics groups interact with the user to ensure robust data that is archived appropriately for future use. This component is key in UNC LCCC's participation in The Cancer Genome Atlas project and serving LCCC needs. Future plans Include expanding staff and equipment so that a full array of Next Gen sequencing and services may be provided in a timely manner, as well as the acquisition of the next generation of technology. The goal of the DNA Sequencing component is to produce high quality sequencing and genotyping data rapidly and at a reasonable cost for UNC-CH researchers using state-of-the-art technologies as well as provide technical support to enhance the value of results produced. Services include DNA sequencing and microsatellite genotyping. The component adds value to the cancer center by producing high-quality data at a competitive price while providing the benefits of a local resource to assist in: scientific strategies, sample preparation, sequencing through difficult regions, and assistance in data interpretation. This component has a wide user base with over 220 different laboratories as users in 2009. Future plans include continued use of existing equipment, reduction of chemistry costs, and evaluation of future sequencing technology options such as long read single molecule sequencing technologies. The goal of the High Throughput Genotyping component is to produce high-quality genotyping data rapidly and at a reasonable cost for researchers using state-of-the-art technologies. Services include SNP genotyping, copy number variation and DNA methylation profiling analysis. New technologies and/or applications are being explored to increase the core's competitive edge and to allow for Genome Wide Association Study on rare variants. These core components are used by multiple members and in each core, peer reviewed member used exceeds 70%. The proposed budget of $241,797 is less than 5% of the core's operating cost. The budget provides salary stability for vital core staff who work with members to adopt these novel technologies.