Core B: Cell Culture and Molecular Technology

SDRC细胞培养和分子技术核心(CCMTC)提供成熟的、具有良好特性的 皮肤细胞系和原代培养，以及系统生物学方法方面的服务和培训 皮肤细胞的研究。核心根据调查人员的需求不断发展，并提供最新的 艺术服务、培训、设施和专业知识。CCMTC已发展成为一个主要的研究中心 在校园内开展活动，并在引入新的研究人员从事皮肤病工作方面发挥了积极作用。 该中心致力于向研究人员提供广泛的培养皮肤细胞，并提供 细胞培养方法学和新兴分子和系统生物技术方面的培训。三位一体 CCMTC的主要任务如下。具体目标1：提供具有成本效益的服务和培训 在最先进的细胞培养和分子技术方面促进与皮肤相关的研究。活动清单 包括提供培养的表皮和真皮细胞(角质形成细胞、黑素细胞、成纤维细胞和 以高效、质量受控和成本效益高的方式向国家发改委调查人员提供免疫细胞)。规定 广泛的分子生物技术(例如，siRNA方法学， 逆转录病毒永生化、基于载体的基因传递等)。具体目标2：向国家发改委提供调查人员 凭借针对特定皮肤的Omics和系统生物学数据分析、咨询、技术和软件 促进涉及皮肤研究的核心间翻译研究和跨学科项目的“管道”。 CCMTC作为皮肤特异性转录组学和蛋白质组学的知识库，并提供 在这些新兴技术的规划、设计和生物信息学分析方面具有专业知识。具体目标3： 促进职业发展，强调以皮肤为中心的研究住院医师、研究员和教职员工 通过帮助成员学习最先进的细胞培养和分子技术来构建身体 研究项目的初步数据。由于调查人员的需求，CCMTC现在将提供几个新的 技术包括皮肤细胞/组织的代谢组分析，基因表达谱的DASL分析 从石蜡包埋组织和全基因组甲基化用于表观遗传学研究。

The SDRC Cell Culture and Molecular Technology Core (CCMTC) provides established, well characterized skin cell lines and primary cultures, as well as services and training in systems biology approaches to the study of skin cells. The core continuously evolves depending on investigator needs and provides state-of-the- art services, training, facilities and expertise. The CCMTC has developed into a major hub of research activity on campus and has an active role in introducing new investigators to work on cutaneous disease. The core is heavily engaged in providing a wide range of cultured skin cells to investigators, and in providing training in cell culture methodology and emerging molecular and systems biology technology. The three major missions of the CCMTC are listed below. Specific Aim 1: To provide cost-effective service and training in state-of-the-art cell culture and molecular technology to promote skin-related research. List of activities include the provision of cultured epidermal and dermal cells (keratinocytes, melanocytes, fibroblasts and immunocytes) to SDRC investigators in an efficient, quality-controlled, and cost effective manner. Provision of, access to and training in a wide range of molecular biologic technologies (e.g., siRNA methodology, retroviral immortalization, vector-based gene delivery, etc.). Specific Aim 2: To provide SDRC investigators with skin-specific 'Omics and systems biology data analysis, consultation, technologies and software to facilitate a "pipeline" of inter-core translational research and interdisciplinary projects involving skin research. The CCMTC serves as a knowledge base for skin-specific transcriptomics and proteomics and provides expertise with the plan, design and bioinformatics analysis of these emerging technologies. Specific Aim 3: To promote career development with a skin-centered emphasis for research residents, fellows and faculty members by helping them to learn state of the art cell culture and molecular techniques as they build a body of preliminary data for research projects. Due to investigator demand, the CCMTC will now offer several new technologies including metabolome analysis from skin cells/tissue, DASL assay for gene expression profiles from paraffin-embedded tissue, and whole genome methylation for epigenetic studies.