Systems Biology Core

系统生物学核心

• 批准号: 8319620
• 负责人: Pratima Karnik
• 金额: $ 6.66万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8319620
• 关键词: Area; Bioinformatics; Biomedical Research; Biopsy; Cellular biology; Clinical; Complement; Computer Systems; Consultations; Data; Family; Floor; Functional disorder; Future; Genomics; Goals; Individual; Information Management; Laboratories; Lasers; Microscopy; Molecular Biology; Molecular Genetics; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Nucleic Acids; Pathway Analysis; Proteins; Proteomics; Psoriasis; RNA; Research; Research Personnel; Resources; Services; Skin; Systems Biology; Techniques; Technology; Testing; Therapeutic Intervention; Time; Tissues; Training; Translational Research; Vertebral column; Walking; Work; data mining; data reduction; design; experience; gene discovery; multidisciplinary; new technology; square foot; success

The theme of this Center of Research Translation (CORT) application is psoriasis. The goal of this multidisciplinary effort is to better understand the pathophysiology of psoriasis, to test therapeutic interventions for psoriasis, and to develop new technologies for treatment of psoriasis. Therefore we include the Systems Biology Core (SBC), designed to support the individual RO1 projects described here. The SBC will provide a variety of services that are targeted to enhance the scientific quality of skin-related research to CORT investigators. The SBC is an integrated molecular biology laboratory focusing on high- throughput gene discovery and analysis, including molecular genetics, genomics and proteomics technologies together with associated bioinformatics and information management. The SBC will provide technological services, training and consultation in state of the art molecular biology technologies such as RNA and protein extraction from small tissue biopsies, laser capture microscopy, real- time PCR quantification of nucleic acids, microarray and proteomics technology and bioinformatics support for management, analysis, display and integration of genomic and proteomics data. Dr. Karnik's laboratory constitutes the backbone of the Systems Biology Core laboratory and will include"! ,000 square feet of research area located on the 5th floor of the Biomedical Research Building. It is equipped for cell and molecular biology studies, an area designated for RNA work and a sophisticated computer system for microarray and proteomics data reduction, data mining and pathway analysis.The SBC will complement and interdigitate with the institutional proteomics and genomics cores as well as with the SDRC cores and the Murdough Family Center of Psoriasis' Regional Clinical Network to facilitate the research of CORT investigators. All facilities are within a five-minute walk in adjacent buildings connected by an indoor bridge. Together, the Systems Biology Core director and consultants have extensive experience with the techniques and services proposed by the Core and will be a great asset to the CORT RO1 projects proposed, as well as to future P&F projects. The SBC will support all of the projects proposed in the CORT and is thus a key resource for success for the Case Psoriasis CORT.

该研究翻译中心（CORT）应用程序的主题是牛皮癣。目标的目标 多学科的努力是更好地了解牛皮癣的病理生理学，以测试治疗 牛皮癣的干预措施，并开发用于治疗牛皮癣的新技术。因此，我们包括 系统生物学核心（SBC），旨在支持此处描述的单个RO1项目。 SBC将提供各种服务，以提高与皮肤相关的科学质量 对Cort研究人员的研究。 SBC是一个集成的分子生物学实验室，专注于高级 吞吐基因发现和分析，包括分子遗传学，基因组学和蛋白质组学 技术以及相关的生物信息学和信息管理。 SBC将在最先进的分子生物学状态下提供技术服务，培训和咨询 从小组织活检中提取RNA和蛋白质等技术，激光捕获显微镜，实地 核酸，微阵列和蛋白质组学技术和生物信息学支持的时间PCR定量 用于管理，分析，显示和集成基因组和蛋白质组学数据。 卡尼克博士的实验室构成了系统生物学核心实验室的骨干，将 包括“！ 配备了细胞和分子生物学研究，该区域指定为RNA工作和复杂的 用于微阵列和蛋白质组学数据降低，数据挖掘和途径分析的计算机系统。SBC 将与制度蛋白质组学和基因组学核心以及 SDRC核心和牛皮癣区域临床网络的Murdough家族中心促进 Cort研究人员的研究。所有设施都在相邻建筑物连接的相邻建筑物中步行五分钟之内 在室内桥上。系统生物学核心主管和顾问共同拥有广泛的 有核心提出的技术和服务的经验，将成为Cort的重要资产 RO1项目以及未来的P＆F项目。 SBC将支持所有提议的项目 在Cort中，这是牛皮癣Cort成功的关键资源。