Systems Biology Core

这项研究翻译中心(CORT)应用的主题是牛皮癣。这样做的目的是 多学科的努力是为了更好地了解牛皮癣的病理生理学，测试治疗方法 对牛皮癣的干预，并开发治疗牛皮癣的新技术。因此，我们包括 系统生物学核心(SBC)，旨在支持此处描述的各个RO1项目。 SBC将提供各种服务，旨在提高皮肤相关的科学素质 给CORT的调查人员做研究。SBC是一家专注于高分子生物学的综合分子生物学实验室。 吞吐量基因发现和分析，包括分子遗传学、基因组学和蛋白质组学 技术以及相关的生物信息学和信息管理。 SBC将提供最先进的分子生物学技术服务、培训和咨询 从小组织活检中提取RNA和蛋白质的技术，激光捕获显微镜，真正的 核酸的时间聚合酶链式反应定量、微阵列和蛋白质组学技术及生物信息学支持 用于基因组和蛋白质组学数据的管理、分析、显示和集成。 卡尼克博士的实验室构成了系统生物学核心实验室的骨干，将 包括“位于生物医学研究大楼5楼的1000平方英尺的研究区域。它是 配备了用于细胞和分子生物学研究的设备，一个指定用于RNA工作的区域和一个复杂的 用于微阵列和蛋白质组学数据简化、数据挖掘和通路分析的计算机系统 将与机构蛋白质组学和基因组学核心以及 国家疾病预防控制中心和墨尔道家族银屑病区域临床网络促进 CORT调查员的研究。所有设施都位于相邻建筑中，步行5分钟即可到达 在一座室内桥旁。总而言之，系统生物学核心总监和顾问拥有广泛的 拥有CORE建议的技术和服务的经验，这将是CORT的一笔巨大财富 建议的RO1项目以及未来的P&F项目。SBC将支持所有拟议的项目 因此，它是治疗牛皮癣成功的关键资源。

The theme of this Center of Research Translation (CORT) application is psoriasis. The goal of this multidisciplinary effort is to better understand the pathophysiology of psoriasis, to test therapeutic interventions for psoriasis, and to develop new technologies for treatment of psoriasis. Therefore we include the Systems Biology Core (SBC), designed to support the individual RO1 projects described here. The SBC will provide a variety of services that are targeted to enhance the scientific quality of skin-related research to CORT investigators. The SBC is an integrated molecular biology laboratory focusing on high- throughput gene discovery and analysis, including molecular genetics, genomics and proteomics technologies together with associated bioinformatics and information management. The SBC will provide technological services, training and consultation in state of the art molecular biology technologies such as RNA and protein extraction from small tissue biopsies, laser capture microscopy, real- time PCR quantification of nucleic acids, microarray and proteomics technology and bioinformatics support for management, analysis, display and integration of genomic and proteomics data. Dr. Karnik's laboratory constitutes the backbone of the Systems Biology Core laboratory and will include"! ,000 square feet of research area located on the 5th floor of the Biomedical Research Building. It is equipped for cell and molecular biology studies, an area designated for RNA work and a sophisticated computer system for microarray and proteomics data reduction, data mining and pathway analysis.The SBC will complement and interdigitate with the institutional proteomics and genomics cores as well as with the SDRC cores and the Murdough Family Center of Psoriasis' Regional Clinical Network to facilitate the research of CORT investigators. All facilities are within a five-minute walk in adjacent buildings connected by an indoor bridge. Together, the Systems Biology Core director and consultants have extensive experience with the techniques and services proposed by the Core and will be a great asset to the CORT RO1 projects proposed, as well as to future P&F projects. The SBC will support all of the projects proposed in the CORT and is thus a key resource for success for the Case Psoriasis CORT.