Resolution Limits for Fluorescence Tomographic Molecular Imaging

描述(由申请人提供)：我们建议进行研究，以确定使用过采样测量的荧光漫反射光学层析成像(FDOT)的分辨率极限。它将利用NCRR National Resources的两项综合专长：使用杜克大学活体显微镜(CIVM)中心最近建立的分子成像工作台获取数据，以及使用匹兹堡超级计算机中心(PSC)的并行计算设施进行图像重建。具体地说，我们将进行以下研究：(I)开发用于FDOT模拟和图像重建的模块化开源软件包；(Ii)确定成像模体的FDOT分辨率极限；以及(Iii)利用先前的解剖学信息确定分辨率极限，并将该技术应用于小鼠肺癌成像。 公共卫生相关性(由申请人提供)：这项提案的目标是调查小动物3D荧光成像的分辨率限制。还将研究来自Micro-CT的解剖先验信息的影响。图像重建将基于蒙特卡罗方法，使用超级计算机上的并行计算。

DESCRIPTION (provided by applicant): We are proposing studies to determine the resolution limits for fluorescence diffuse optical tomography (FDOT) using over-sampled measurements. It will exploit the combined expertise of two NCRR National Resources: data acquisition using the recently established Molecular Imaging Workbench at the Duke Center for In Vivo Microscopy (CIVM) and image reconstruction using the parallel computing facilities at the Pittsburgh Supercomputer Center (PSC). Specifically, we will be conducting the following studies: (i) Develop a modular open-source software package for FDOT simulations and image reconstruction; (ii) Determine FDOT resolution limits in imaging phantoms; and (iii) Determine the resolution limits with prior anatomical information and apply the technology to lung cancer imaging in mice. PUBLIC HEALTH RELEVANCE (provided by applicant): The goal of this proposal is to investigate the resolution limit in 3D fluorescence imaging for small animals. The effects of anatomical prior information from micro-CT will also be studied. Image reconstruction will be based on a Monte Carlo method using parallel computing on supercomputers.