Core C: Sphingolipid Cancer Animal Pathobiology Core

项目总结 鞘磷脂动物癌症病理生物学(SACP)共享资源核心的总体目标是 使项目负责人能够利用动物致癌模型执行其 研究项目，并维持基因工程小鼠的致癌模型。《SACP》 共享资源核心将作为使用以下各项所需的所有动物操作的基本资源 这些项目。通过集中体内研究的所有方面，SACP核心将促进更快速和 可靠的数据，并最大限度地减少使用动物模型的成本。这项建议的总体目标是： 具体目标1：确保适当规划活体研究模型，提供必要的设施和 提供致癌模型方面的专业知识，并促进关键共享资源的使用。我们将a)协助 研究人员选择和/或设计最合适的动物模型来检验假设，包括 基于脂肪的转基因和基因改变小鼠：b)提供必要的设施以及教职员工 支持致癌动物模型研究项目的专门知识；以及c)便利获取和使用 SBU现有的共享资源，包括小动物成像、药物代谢和药代动力学， 组织生物库以及转基因和基因敲除产生设施。具体目标2：加强和 最大限度地发挥动物模型的效用，并提供补充和替代方法。为了这个 目的：我们将a)为项目调查员维持饲养动物的数量；b)协助利用 致癌模型；以及c)为项目研究人员提供动物研究的替代方案 来自基于脂质的基因工程小鼠的细胞系。具体目标3：向调查人员提供 在数据收集、解释、分析和管理方面提供协助和专业知识。在《目标3》中，我们将 A)向调查人员提供病理学、动物模型病理生物学和免疫组织化学方面的专门知识；b) 协助数据分析、解释和项目进展；以及c)促进样品储存，以备将来使用 致癌动物模型的研究。 SACP核心的关键人员包括一名经验丰富的病理学家和一名生物库科学主管。 这些服务将提供必要的啮齿动物病原学和相关的病理生物学方面的专业知识。 将致癌模型添加到从动物模型产生的生物库样本中 致癌。这些努力将促进本提案中的研究，并将提供专门知识 支持项目负责人。这一核心已经演变为一个独特的、支持的核心，这对 计划项目的成功。

PROJECT SUMMARY The overall objective of the Sphingolipid Animal Cancer Pathobiology (SACP) Shared Resource Core is to provide the Project Leaders with the ability to utilize animal models of carcinogenesis in the execution of their research projects and to maintain genetically engineered mice for the carcinogenesis models. The SACP Shared Resource Core will serve as an essential resource for the use of all animal manipulations needed by the Projects. By centralizing all aspects of in vivo research, the SACP Core will facilitate more rapid and reliable data and minimize the cost of utilizing animal models. The overall aims of the proposal are: Specific Aim 1: Ensure proper planning of in vivo research models, provide necessary facilities and expertise for carcinogenesis models and facilitate use of critical Shared Resources. We will a) assist investigators with choosing and/or designing the most appropriate animal model to test hypotheses, including lipid-based transgenic and gene-altered mice: b) provide essential facilities as well as faculty and staff expertise to support research projects in animal models of carcinogenesis; and c) facilitate access to and use of existing shared resources at SBU, including small animal imaging, drug metabolism and pharmacokinetics, tissue biorepository and transgenic and gene knockout generation facilities. Specific Aim 2: Enhance and maximize the utility of animal models and provide complementary and alternative approaches. For this Aim we will a) maintain stocks of breeding animals for the Project investigators; b) assist with utilization of carcinogenesis models; and c) provide Project investigators with alternatives to animal research via generation of cell lines from lipid-based genetically engineered mice. Specific Aim 3: Provide investigators with assistance and expertise in data collection, interpretation, analysis and management. In Aim 3 we will a) provide investigators with expertise in pathology, animal model pathobiology and immunohistochemistry; b) assist with data analysis, interpretation and project progression; and c) facilitate sample storage for future studies in animal models of carcinogenesis. Key personel for the SACP Core include an experienced pathologist and a scientific director for Biobanking. Together, these services will provide the necessary expertise in rodent patholgoy and pathobiology associated with carcinogenesis models in additon to biobanking samples generated from animal models of carcinogenesis. These efforts will facilitate the research in this proposal and will provide the expertise to support the Project Leaders. This core is already evolving as a unique and enabling core that is critical for the success of the Program Project.