ANIMAL PATHOBIOLOGY CORE

动物病理生物学核心的总体目标是为中心的研究人员提供在执行其拟议的研究项目中利用动物模型的能力，以及建立新的动物模型来研究疾病的发病机制。核心是脂肪组学和病理生物学中心翻译研究发展的独特和不可或缺的一部分，也是唯一包含与脂质相关的动物模型的独特汇编的设施。在超过25只转基因小鼠中，有11种鞘脂代谢酶的敲除菌株，3种鞘脂受体的敲除菌株，4种与鞘脂代谢相关的敲除菌株，以及7种鞘脂或鞘脂相关蛋白的转基因菌株。动物病理生物学核心还获得并促进了新的转基因动物模型的创建；包括通过与MUSC现有的其他现有共享资源协调来生成针对基因的和缺失的动物。这些动物模型随后由Core饲养、繁殖和回交，以方便中心调查人员使用。除了为项目提供动物/模型外，核心还提供规划、执行和数据解释方面的专业知识。最后，核心提供教程，并促进用户在动物研究的各个方面的教育。因此，这种集中的资源使中心的研究人员能够使用设备、专业知识和动物资源，使动物研究变得更加可行、更容易获得和更具成本效益。通过专注于鞘磷脂相关的靶点和相关的病理生物学过程，Core正在成为使中心研究人员能够进行临床前翻译研究的关键组成部分。

The overall objective of the Animal Pathobiology Core is to provide the Center investigators with the ability to utilize animal models in the execution of their proposed research projects as well as establish new animal model for the investigation of the mechanisms involved in the pathogenesis of disease. The Core is a unique and integral part of the translational research development of the Center for Lipidomics and Pathobiology and is the only facility that encompasses a unique compilation of lipid-related animal models. Encompassed in the more than 25 genetically altered mice are knockout strains for 11 sphingolipid metabolizing enzymes, 3 for the sphingolipid receptors, and 4 related to sphingolipid metabolism, as well as 7 transgenic strains for sphingolipid or sphingolipid-related proteins. The Animal Pathobiology Core also obtains and facilitates the creation of novel genetically altered animal models; including generation of gene targeted and deleted animals through coordination with other existing shared resources available at MUSC. These animal models are then housed, bred and backcrossed by the Core to facilitate their use by Center investigators. In addition to animals/models for projects, the Core provides expertise in planning, execution and data interpretation. Finally, the Core provides tutorials and facilitates education of users in various aspects of animal research. Thus, this centralized resource enables Center investigators in the use of equipment, expertise and animal resources making animal research more feasible, accessible and cost effective. By focusing on sphingolipid-related targets and associated pathobiologic processes, the Core is emerging as a key component enabling pre-clinical translational research for Center investigators.