UVA molecular electron microscopy core for the Mid-Atlantic region

摘要 弗吉尼亚大学（UVa）的分子电子显微镜核心（MEMC）设施包括： 三个透射电子显微镜。FEI 120 kV Spirit位于Snyder的二楼 方丹研究园的转化研究大楼。FEI 200 kV F20和300 kV Titan Krios 位于地下生命科学附件（丽莎）的一个专门设计的套房内，毗邻并连接 斯奈德大厦预计未来高分辨率cryoEM研究的增长，该套件的目的是 可容纳两台Titan Krios电子显微镜泰坦克里奥斯有一个自动装弹机， 一次可操作多达12个冷冻滤线栅，使用恒流透镜以提高稳定性， 配备了XFEG以改善照明和Falcon II直接电子探测器。2009年，抽象 我们的G20赠款，支持了丽莎套件的建设指出，“MEMC设施将（1）提高 在UVa的研究质量，（2）提高科学生产力，（3）促进沟通，互动和 科学家之间的合作，（4）提高UVa吸引和维持一流教师的能力， （5）加强学院的竞争力。MEMC设施不仅将 提供给医学院的教职员工，也提供给其他学校和部门的其他科学家 在弗吉尼亚大学。这将是UVa的一个独特的核心实验室，我们设想整个弗吉尼亚州的科学家和 周边各州将访问弗吉尼亚大学，利用这样一个尖端的实验室进行表演， 分子分辨电子显微镜”我们在UVa有着强烈的合作精神， 欢迎有机会在UVa内外分享MEMC设施。我们相信，我们的愿景 MEMC设施作为大西洋中部地区科学家的最先进的cryoEM中心， 实现了在过去的两年中，UVa的23个实验室和12个外部实验室使用MEMC收集了数据。 14个实验室已经使用Titan Krios收集了数据。一半的可用时间将用于财团PI， 在cryoEM和图像分析方面具有丰富经验的人：Jenny Hinshaw博士（NIH），Deborah Kelly （弗吉尼亚理工大学）、梅勒妮·奥希（范德比尔特）、蒙特塞拉特·萨姆索（VCU）和张培军（皮特）。作为首要 作为MEMC的内部UVa用户，Yeager和Egelman博士拥有超过50年的经验。作为 核心主任凯利德莱登博士与所有用户密切互动。U24补助金，加上 由UVa医学院提供的30万美元/年将使MEMC在财政上可持续。

Abstract The Molecular Electron Microscopy Core (MEMC) facility at the University of Virginia (UVa) is comprised of three transmission electron microscopes. An FEI 120 kV Spirit is housed on the second floor of the Snyder Translational Research Building at Fontaine Research Park. An FEI 200 kV F20 and a 300 kV Titan Krios are located in a specially designed suite in the underground Life Sciences Annex (LiSA) adjacent and connected to the Snyder Building. Anticipating future growth in high-resolution cryoEM research, the suite was designed to accommodate two Titan Krios electron microscopes. The Titan Krios has an autoloader that robotically manipulates up to 12 frozen grids inserted at one time, uses constant current lenses for greater stability, is equipped with an XFEG for improved illumination and a Falcon II direct electron detector. In 2009, the abstract of our G20 grant that supported the build-out of the LiSA suite stated, “The MEMC facility will (1) enhance the quality of research at UVa, (2) increase scientific productivity, (3) foster communication, interactions and collaboration amongst scientists, (4) boost the ability of UVa to attract and maintain premier faculty and students, and (5) strengthen the competitiveness of our faculty for funding. The MEMC facility will not only be available to School of Medicine faculty members but also to other scientists in other schools and departments at UVa. This will be a unique Core laboratory at UVa, and we envision that scientists throughout Virginia and the surrounding states will visit UVa to take advantage of such a cutting-edge laboratory for performing molecular resolution electron microscopy.” We have a strong spirit of collegiality and collaboration at UVa and welcome the opportunity to share the MEMC facility within UVa and beyond. We believe that our vision of the MEMC facility as a state-of-the-art cryoEM center for scientists in the mid-Atlantic region has already been realized. Over the last two years, 23 labs at UVa and 12 external labs have collected data using the MEMC. 14 labs have collected data using the Titan Krios. Half of the available time will be devoted to Consortium PIs, who have substantial experience in cryoEM and image analysis: Drs. Jenny Hinshaw (NIH), Deborah Kelly (Virginia Tech), Melanie Ohi (Vanderbilt), Montserrat Samso (VCU), and Peijun Zhang (Pitt). As the primary internal UVa users of the MEMC, Drs. Yeager and Egelman have more than 5 decades of experience. As Director of the Core, Dr. Kelly Dryden interacts closely with all users. The U24 grant, combined with $300K/year provided by the UVa School of Medicine, would make the MEMC financially sustainable.