Advanced Imaging and Tissue Analysis Core

项目概要AITAC WU-DDRCC的首要使命是促进合作，多学科研究，重点是 消化系统疾病中宿主与环境的相互作用AITAC支持组织成像， 分析，用于可视化不同细胞类型和亚细胞组分在细胞中的空间关系的工具。 肝脏和胃肠道。AITAC人员在形态分析和成像方面具有广泛的专业知识 消化组织。自2013年以来，AITAC提供了全面，先进的显微成像 每年为45个正式成员和26个准成员（>90%的成员）提供图像分析服务， 高效、经济的方式。AITAC配置有两个组件：（1）。组织分析组件， 它进行组织包埋，切片和免疫组织化学分析，并提供所有培训， 服务组织分析组件（光学和荧光显微镜）位于单个核心实验室中， 还提供了一个单独的光控制室，仅用于显微镜检查。（二）、高级图像分析组件， 提供以下方面的机会和培训：（i）常规光、荧光和倒置荧光显微镜检查， 定量图像分析（ii）进入机构体内成像核心（IVIC），一个由2- 光子显微镜和设备，具有完整的活细胞、体内器官和组织成像技术支持;以及 (iii)访问华盛顿大学细胞成像中心（WUCCI; http：//wucci.wustl.edu/）， 设施提供先进的细胞成像技术，包括共聚焦，超分辨率，传输，光 薄片、扫描电子显微镜和纳米断层摄影术。主要成就包括： 显微镜和图像分析用途;投资购买了2台新显微镜（Olympus BX 43 F光学显微镜， 高分辨率成像和奥林巴斯SZX-ZB 7立体变焦解剖显微镜）;升级的蔡司图像 分析软件;安装了全自动载玻片。我们在周年调查中回应了议员的需要， 扩展成像和图像分析组件，并通过扩展 我们的补充奖励计划（详见行政（ARAC）核心报告）。的总体影响 自2000年以来，这些成就已经导致156份原创科学出版物引用了WU-DDRCC 2013.基于这些成就和目标，我们目前的具体目标包括：（1）。以提供高度 具有成本效益的全面组织分析服务和广泛组织学领域的研究者培训 程序，包括人类和研究动物组织处理、包埋、常规和特殊染色 和免疫组化分析，促进我们对消化系统疾病病理生理学的理解。 （二）、提供使用高级成像和图像分析工具（如蔡司）的机会和培训 Axiovert，配备Apotome、Zeiss和InForm图像分析软件，可使用机构双光子显微镜 用于体内实时成像（IVIC），并访问WUCCI进行高级成像，包括3D和冷冻电子成像 显微镜;超分辨率和X射线显微镜。

PROJECT SUMMARY AITAC The overarching mission of the WU-DDRCC is to promote collaborative, multidisciplinary research focused on interactions between host and environment in digestive disease. The AITAC supports tissue imaging and analysis, tools for visualizing the spatial relationships of different cell types and subcellullar components in the liver and gastrointestinal tract. AITAC personnel have extensive expertise in morphologic analysis and imaging of digestive tissues. Since 2013, the AITAC has provided comprehensive, advanced microscopic imaging and image analysis services to 45 Full and 26 Associate members (>90% members) per year in an efficient, cost-effective manner. AITAC is configured with two components: (1). Tissue Analysis Component, which performs tissue embedding, sectioning, and immunohistochemical analyses, and provides training in all services. The Tissue Analysis Component (light and fluorescence microscopes) reside in a single core lab which also provides a separate light-controlled room for microscopy only. (2). Advanced Image Analysis Component, which provides access to and training in (i) routine light, fluorescent and inverted fluorescent microscopy and quantitative image analysis (ii) access to the institutional In Vivo Imaging Core (IVIC), an investigator-built 2- photon microscope and facility with complete technical support for live cell, in vivo organ and tissue imaging; and (iii) access to the Washington University Center for Cellular Imaging (WUCCI; http://wucci.wustl.edu/) this facility provides advanced cellular imaging technologies including confocal, super-resolution, transmission, light sheet, scanning electron microscopy and nanotomography. KEY ACCOMPLISHMENTS include:increased microscope and image analysis usage; invested in 2 new microscopes (an Olympus BX43F light microscope for high-resolution imaging and an Olympus SZX-ZB7 stereo zoom dissecting microscope); upgraded Zeiss image analysis software; installed a fully automated slide. We responded to Members' needs in annual surveys by expanding imaging and image analysis components, and leveraged access to the WUCCI by expanding our Supplemental Awards program (detailed in Administrative (ARAC) Core report). The overall impact of these accomplishments has resulted in 156 original scientific publications citing the WU-DDRCC since 2013. Based on these accomplishments and goals, our current SPECIFIC AIMS include: (1). To provide highly cost-effective, comprehensive tissue analysis services and investigator training in an extensive array of histologic procedures, including human and research animal tissue processing, embedding, routine and special staining and immunohistochemical analyses that advance our understanding of digestive disease pathophysiology. (2). To provide access to and training in the use of advanced imaging and image analysis tools such as the Zeiss Axiovert with Apotome, Zeiss and InForm image analysis software, access to institutional 2-photon microscopy for in vivo, live imaging (IVIC) and access to the WUCCI for advanced imaging including 3D and cryo-electron microscopy; super-resolution and x-ray microscopy.