Core-003: Histology Core

组织学核心项目摘要/摘要 肌肉骨骼组织的结构和组成是为满足其要求的功能而量身定做的。 随着损伤、衰老和特定疾病的发生，这些组织的结构和组成会恶化，导致 生物、表型和机械功能和特性的衰退或丧失。肌肉骨骼组织 每一种都具有相对于细胞成分的广泛的组成和结构变化， 微体系结构和细胞外基质成分。仔细描述和量化组织 结构组织和组成，以及分泌因子的定位和识别 阐明肌肉骨骼完整性、损伤、 并进行修复。该组织学核心(HC)的总体目标是利用和开发广泛的标准 和创新的组织学和组织形态计量学方法来评估肌肉骨骼组织损伤和 维修，并利用这些检测为新项目和合作项目提供培训和资金。 对专门的组织学技术的需求越来越大，新的 以肌肉骨骼为基础的组织形态方法和组织学方法的定制 有问题。由于认识到许多肌肉骨骼 疾病可以影响其他系统(间接地，包括肌肉、骨骼和其他连接结构)。在……里面 此外，一些病理是真正的多系统疾病，也会影响肌肉骨骼组织。因此， 最初关注非肌肉骨骼病理学的调查人员现在也需要访问 由医管局提供的意见和服务。此外，随着新的动物模型的激增，它是 关键是能够表征他们的肌肉骨骼表型，无论是主要的还是次要的 受遗传疾病或其他类型的肌肉骨骼侮辱的影响。宾夕法尼亚州立大学医学中心 肌肉骨骼疾病(PCMD)在罗伯特·皮格诺洛博士的领导下蓬勃发展，他一直 自2010年7月起担任董事。重要的是，HC的家在Stemmler Hall，和其他两个人一样 资源核心和管理核心，总体上为PCMD定义了一个明确的家。 人力资源中心的具体目标是：1)提供有关能力、优势、 评估肌肉骨骼组织结构和组成的各种方法的缺点 通过正式的教育丰富计划和一对一的互动，2)提供高质量的 肌肉骨骼组织的组织学和组织形态计量学分析的专业知识和服务，3)开发 将适用于肌肉骨骼研究的基于组织学的新的创新技术，以及4) 为新项目的开发和合作提供资金，并开发初步和/或 为调查人员提供可行性数据。

Project Summary/Abstract for Histology Core The structure and composition of musculoskeletal tissues are tailored to meet their demanding functions. With injury, aging, and specific diseases, the structure and composition of these tissues deteriorate, resulting in a decline or loss of biological, phenotypic and mechanical function and properties. Musculoskeletal tissues each have a wide array of compositional and structural variety with respect to cellular components, microarchitecture, and extracellular matrix constituents. Careful description and quantification of tissue structural organization and composition, as well as localization and identification of secreted factors, are necessary requirements for elucidation of the biologic mechanisms underlying musculoskeletal integrity, injury, and repair. The overall objective of this Histology Core (HC) is to utilize and develop a wide range of standard and innovative histological and histomorphometric approaches to evaluate musculoskeletal tissue injury and repair, and to provide training and funding for new projects and collaborations utilizing these assays. There is an increasing demand for specialized histological techniques, development of new histomorphological methods, and customization of histological approaches to musculoskeletal-based problems. The need for these approaches is ever-broadening due to the recognition that many musculoskeletal disorders can affect other systems (indirectly, including muscle, bone, and other connective structures). In addition, some pathologies are true multisystem disorders that also affect musculoskeletal tissues. Thus, investigators whose interests were initially focused on non-musculoskeletal pathology now also require access to the advice and services provided by the HC. In addition, with the proliferation of new animal models, it is critical to be able to characterize their musculoskeletal phenotype, whether it is primarily or secondarily affected by a genetic disorder or other type of musculoskeletal insult. The HC of the Penn Center for Musculoskeletal Disorders (PCMD) has flourished under the leadership of Dr. Robert Pignolo, who has been Director since July 2010. Importantly, the home of HC is in Stemmler Hall, the same building as the other two Resource Cores and the Administrative Core, defining a clear home for the PCMD overall. The Specific Aims of the HC are to: 1) To provide guidance and training on the capabilities, advantages, and disadvantages of the various methodologies to assess musculoskeletal tissue structure and composition through formal educational enrichment programs and one-on-one interactions, 2) To provide high-quality expertise and service for histological and histomorphometric assays of musculoskeletal tissues, 3) To develop new and innovative histologically-based techniques that will be applicable to musculoskeletal research, and 4) To provide funding for development of new projects and collaborations and to develop preliminary and/or feasibility data for investigators.