Antibody Core

项目摘要：抗体核心 《催产素对神经回路功能的调节》这一脑部首创提案的主要特点之一 和行为“是催产素受体抗体产生的研究支持核心。每个项目团队 正在研究催产素的释放和催产素受体信号的作用。催产素是最好的- 了解生理和行为水平的调节器，多年来一直以各种形式进行研究 一个世纪。然而，催产素信号对细胞和网络的影响知之甚少，部分原因是 以前缺乏特异性抗体来确定哪些大脑区域和细胞类型表达催产素受体。 我们的实验室已经产生、验证并正在分发第一批针对小鼠催产素受体的特异性抗体， 鉴于科学界的成功使用和热情，我们感到有义务扩大生产 并改进它们的功能。抗体核心的目标1是继续产生催产素 受体抗体，目标2是广泛分布这些抗体，目标3是产生单克隆 以方便美国和国际上的项目实验室和其他研究人员更广泛地使用这些抗体。 显然迫切需要这些资源，这些资源最好由一个核心来生产、测试和分发 而不是单独的实验室。

Project Summary: Antibody Core One of the major features of this BRAIN Initative proposal on “Oxytocin Modulation of Neural Circuit Function and Behavior” is this Oxytocin Receptor Antibody Production Research Support Core. Each of the Project teams is examining oxytocin release and the action of oxytocin receptor signaling. Oxytocin is one of the best- understood modulators at the physiological and behavioral levels, having been studied in various forms for over a century. However, little is known about the cellular and network effects of oxytocin signaling, in part due to previous lack of specific antibodies for determining which brain areas and cell types express oxytocin receptors. Our labs have generated, validated, and are distributing the first specific antibodies to mouse oxytocin receptors, and given the successful use and enthusiasm by the scientific community, we feel obliged to scale-up production of these reagents and improve their functionality. Aim 1 of the Antibody Core is to continue production of oxytocin receptor antibodies, Aim 2 is to distribute these antibodies broadly, and Aim 3 is to generate monoclonal antibodies to facilitate their broader use by Project labs and other investigators both in the US and internationally. There is clearly urgent need for these resources, which are best produced, tested, and distributed by a Core facility rather than by individual labs.