High Sensitivity sCMOS Camera System for Transmission Electron Microscope

项目摘要/摘要 这项提案代表了一组由美国国立卫生研究院资助的研究人员提出的请求，他们的成像需求重叠 资助购置新的高灵敏度cmos相机，以促进蜂窝沙子的可视化。 纳米级的细胞超微结构。摄像机将安装在现有的传输电子设备上 已经安装在华盛顿大学细胞成像中心(WUCCI)的显微镜； 以医学院为基地的全机构共享技术资源。安装在的当前摄像头 WUCCI中的TEM已超过15年，开始遭受越来越多的死像素和 没有提供促进主要和次要用户项目工作所需的信噪比 在此描述的。AMT NanoSprint 15 Mk-II是一款全新的1500万像素闪烁cmos摄像系统 在多种不同的样品类型上提供无与伦比的信噪比。来自10个国家的13名调查人员 医学院和文理学院的不同部门将利用这一新的和 改进的相机平台，使旨在了解分子的广泛研究成为可能 病毒感染、血管畸形、内分泌功能障碍、神经变性、代谢的机制 动态平衡、轴突再生和机械感觉以及对小说发展的帮助 治疗癌症和炎症的纳米治疗方法。专业知识和机构支持 对于这种乐器来说是非常特殊的。WUCCI科学总监James Fitzpatrick博士和David Piston博士， 细胞生物学与生理学主席和WUCCI顾问委员会主席都是世界知名的专家 在细胞显微镜领域，他们的共同领导带来了40多年的提供成本- 为范围广泛的美国国立卫生研究院提供高质量的定量光学和电子显微镜培训和支持。 资助用户。为了支持这项S10拨款申请，该机构还将承诺10万美元(每年2万美元 五年)，以确保该设备的长期成功。

Project Summary/Abstract This proposal represents a request from a group of NIH-funded investigators with overlapping imaging needs for funding to acquire a new high-sensitivity CMOS camera to facilitate the visualization of both cellular sand sub- cellular ultrastructure at the nanoscale. The camera will be installed on an existing Transmission Electron Microscope that is already housed in the Washington University Center for Cellular Imaging (WUCCI); an institution-wide shared technology resource based at the School of Medicine. The current camera installed on the TEM in the WUCCI is over 15 years old, is starting to suffer from an increasing number of dead pixels and does not provide the signal-to-noise ratios needed to facilitate the work of the Major and Minor User projects described herein. The AMT NanoSprint15 Mk-II is a new 15 Megapixel scintillated CMOS camera system providing unparalleled signal-to-noise across many different sample types. Thirteen investigators from ten different departments across the Schools of Medicine and Arts & Sciences will make use of this new and improved camera platform to enable a wide-range of research studies aimed at understanding the molecular mechanisms of viral infection, vascular malformation, endocrine dysfunction, neurodegeneration, metabolic homeostasis, axonal regeneration and mechanosensation as well aid in the development of novel nanotherapeutic approaches to treat cancer and inflammatory conditions. The expertise and institutional support for this instrument are exceptional. Dr. James Fitzpatrick, the Scientific Director of WUCCI, and Dr. David Piston, the Chair of Cell Biology & Physiology and head of the WUCCI Advisory Board, are both world-renowned experts in cellular microscopy and their combined leadership brings over 40 years of experience in providing cost- efficient training and support for high quality quantitative light and electron microscopy to a wide range of NIH- funded users. In support of this S10 grant application, the institution will also commit $100,000 ($20,000 per year for five years) to ensure the long-term success of this equipment.