Epigenetics Core

表观遗传学核心（核心C）提供全基因组DNA甲基化，组蛋白修饰和染色质 免疫沉淀（ChIP）服务，沿着靶向基因区域分析和验证，以支持 方案项目。表观遗传学核心提供服务的直接联系，行政核心A， hESC核心B、计算/生物信息学核心D以及基本的现有UCLA核心，包括 基因表达核心设施（S纳尔逊）。表观遗传学核心得到安捷伦的大力支持 技术（用于阵列平台、探针技术以及分析工具开发和采用）， 支持核心服务的主要顾问，包括C Plass和T Huang（DMA甲基化），M Grunstein 和S Kurdistani（组蛋白修饰）和H Cedar（ChIP）。核心C将提供全基因组或靶向的 hESC和衍生分化细胞中DNA甲基化模式改变的检测和验证 来自hESC核心B和来自程序项目1 - 3。Core C提供ChIP程序、探针 制造、阵列杂交、数据收集和通过将数据传输到计算/分析系统进行分析。 生物信息学核心D.表观遗传学核心将分发信息和协议， 每个计划项目和核心中的人员（根据需要），将发挥有限但重要的作用 在技术开发方面，主要侧重于减少阵列ChIP芯片技术的细胞数量输入 与核心用户和评估/纳入在表观遗传修饰检测的进展，因为他们成为 可通过一般科学界和安捷伦科技公司获得。获得 用于高级ChIP芯片分析的Solexa 1G高通量测序系统通过UCLA 基因表达核心设施（S纳尔逊）。作为一项重要的比较服务， 对于整个hESC群体，表观遗传学核心C将提供全球DNA的基线测量， 甲基化、全乙酰化组蛋白H3和H4以及二甲基化和三甲基化组蛋白， 反映活性或沉默的基因表达，用于最佳生长的低传代联邦批准的hESC系 UC 01（HSF-1）、UC 06（HSF-6）、WA 01（H1）和WA 09（H9）进行比较分析。表达谱 对于这4个联邦批准的系（已经发表或将在现有的UCLA基因中进行）， 表达核心设施）将提供表观遗传标记和对hESC基因的影响之间的联系 表情这些信息将在计划项目的公共访问部分提供 网站，与数据输入和操作密码保护.

The Epigenetics Core (Core C) provides genome-wide DMA methylation, histone modification, and chromatin immunoprecipitation (ChIP) services, along with targeted gene region analysis and validation, to support the Program Projects. The Epigenetics Core provides direct linkage of services to the Administrative Core A, hESC Core B, Computational/Bioinformatics Core D and also to essential existing UCLA cores, including the Gene Expression Core Facility (S Nelson). The Epigenetics Core is strongly supported by Agilent Technologies (for array platforms, probe technologies, and analytical tool development and adoption) and by key consultants that support Core services, including C Plass and T Huang (DMA methylation), M Grunstein and S Kurdistani (histone modification), and H Cedar (ChIP). Core C will provide genome-wide or targeted detection and validation for altered patterns of DNA methylation in hESCs and derivative differentiated cells from the hESC Core B and from Program Projects 1-3. Core C provides ChIP procedures, probe manufacture, array hybridizations, data collection, and analysis through transfer of data to Computational/ Bioinformatics Core D. The Epigenetics Core will distribute information and protocols and help train personnel (as needed) in each of the Program Projects and Cores and will have a limited but important role in technology development, focusing mainly on reducing cell number inputs for array ChlP-chip technologies with Core users and assessing/incorporating advances in epigenetic modification detection as they become available through the general scientific community and in conjunction with Agilent Technologies. Access to the Solexa 1G high throughput sequencing system for advanced ChlP-chip analysis is through the UCLA Gene Expression Core Facility (S Nelson). As an essential comparative service to the Program Project and to the hESC community as a whole, Epigenetics Core C will provide baseline measurements of global DNA methylation, pan-acetylated histone H3 and H4, and di- and tri-methylated histones, epigenetic markings that reflect active or silenced gene expression, for optimally grown low-passage federally-approved hESC lines UC 01 (HSF-1), UC 06 (HSF-6), WA01 (H1), and Wa09 (H9) for comparative analysis. Expression profiling for these 4 federally-approved lines (already published or to be performed in the existing UCLA Gene Expression Core Facility) will provide a link between epigenetic signatures and effects on hESC gene expression. This information will be made available in a publicly accessible portion of the Program Project Website, with data entry and manipulation password protected.