Epigenetics Core

表观遗传学核心

• 批准号: 8379989
• 负责人: MICHAEL A TEITELL
• 金额: $ 18.11万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8379989
• 关键词: Academia; Adopted; Adoption; Area; Bioinformatics; Biological Assay; Cell Count; Cells; Chromatin; Communities; Core Facility; Cytogenetics; DNA; DNA Methylation; Data; Data Collection; Detection; Epigenetic Process; Gene Expression; Gene Expression Profile; Gene Targeting; Genes; Genome; Genomics; Growth; Histone H3; Histone H4; Histones; Human Resources; Hybridization Array; Jonsson Comprehensive Cancer Center of University of California Los Angeles; Letters; Link; Measurement; Methods; Methylation; Modification; Molecular Profiling; Pattern; Procedures; Process; Protocols documentation; Publishing; Role; Sampling; Science; Services; Techniques; Technology; Training; Transcription Initiation Site; Validation; WA01 cell line; advanced system; analytical tool; bisulfite; chromatin immunoprecipitation; comparative; data acquisition; genome sequencing; genome-wide; histone modification; human embryonic stem cell; interest; programs; promoter; technology development; tool development; web site

The Epigenetics Core (Core C) provides genome-wide DMA methylation, histone modification, and chromatin immunoprecipitation (ChIP) services, along with targeted gene region analysis and validation, to support the Program Projects. The Epigenetics Core provides direct linkage of services to the Administrative Core A, hESC Core B, Computational/Bioinformatics Core D and also to essential existing UCLA cores, including the Gene Expression Core Facility (S Nelson). The Epigenetics Core is strongly supported by Agilent Technologies (for array platforms, probe technologies, and analytical tool development and adoption) and by key consultants that support Core services, including C Plass and T Huang (DMA methylation), M Grunstein and S Kurdistani (histone modification), and H Cedar (ChIP). Core C will provide genome-wide or targeted detection and validation for altered patterns of DNA methylation in hESCs and derivative differentiated cells from the hESC Core B and from Program Projects 1-3. Core C provides ChIP procedures, probe manufacture, array hybridizations, data collection, and analysis through transfer of data to Computational/ Bioinformatics Core D. The Epigenetics Core will distribute information and protocols and help train personnel (as needed) in each of the Program Projects and Cores and will have a limited but important role in technology development, focusing mainly on reducing cell number inputs for array ChlP-chip technologies with Core users and assessing/incorporating advances in epigenetic modification detection as they become available through the general scientific community and in conjunction with Agilent Technologies. Access to the Solexa 1G high throughput sequencing system for advanced ChlP-chip analysis is through the UCLA Gene Expression Core Facility (S Nelson). As an essential comparative service to the Program Project and to the hESC community as a whole, Epigenetics Core C will provide baseline measurements of global DNA methylation, pan-acetylated histone H3 and H4, and di- and tri-methylated histones, epigenetic markings that reflect active or silenced gene expression, for optimally grown low-passage federally-approved hESC lines UC 01 (HSF-1), UC 06 (HSF-6), WA01 (H1), and Wa09 (H9) for comparative analysis. Expression profiling for these 4 federally-approved lines (already published or to be performed in the existing UCLA Gene Expression Core Facility) will provide a link between epigenetic signatures and effects on hESC gene expression. This information will be made available in a publicly accessible portion of the Program Project Website, with data entry and manipulation password protected.

表观遗传学核心（核心 C）提供全基因组 DMA 甲基化、组蛋白修饰和染色质 免疫沉淀 (ChIP) 服务以及目标基因区域分析和验证，以支持 计划项目。表观遗传学核心提供与行政核心 A 的直接服务联系， hESC 核心 B、计算/生物信息学核心 D 以及现有的 UCLA 核心，包括 基因表达核心设施（S Nelson）。表观遗传学核心得到安捷伦的大力支持 技术（用于阵列平台、探针技术以及分析工具的开发和采用）以及 支持核心服务的主要顾问，包括 C Plass 和 T Huang（DMA 甲基化）、M Grunstein 和 S Kurdistani（组蛋白修饰）和 H Cedar（ChIP）。 Core C 将提供全基因组或靶向 检测和验证 hESC 和衍生分化细胞中 DNA 甲基化模式的改变 来自 hESC 核心 B 和计划项目 1-3。 Core C 提供 ChIP 程序、探针 通过将数据传输到计算/分析来进行制造、阵列杂交、数据收集和分析 生物信息学核心 D. 表观遗传学核心将分发信息和协议并帮助培训 每个计划项目和核心中的人员（根据需要）将发挥有限但重要的作用 在技​​术开发方面，主要致力于减少阵列 ChLP 芯片技术的细胞数量投入 与核心用户一起评估/整合表观遗传修饰检测的进展 可以通过一般科学界并与安捷伦科技公司合作获得。访问 用于高级 ChlP 芯片分析的 Solexa 1G 高通量测序系统由 UCLA 提供 基因表达核心设施（S Nelson）。作为该计划项目的一项重要比较服务， 表观遗传学 Core C 将向整个 hESC 社区提供全球 DNA 的基线测量 甲基化、全乙酰化组蛋白 H3 和 H4、二甲基化和三甲基化组蛋白、表观遗传标记 反映活跃或沉默的基因表达，用于最佳生长的联邦批准的低传代 hESC 系 UC 01 (HSF-1)、UC 06 (HSF-6)、WA01 (H1) 和 Wa09 (H9) 进行比较分析。表达谱 对于这 4 个联邦批准的细胞系（已经发表或将在现有的 UCLA Gene 表达核心设施）将提供表观遗传特征与 hESC 基因影响之间的联系 表达。此信息将在计划项目的可公开访问的部分中提供 网站，数据输入和操作受密码保护。