CORE--TISSUE CULTURE, MICROSCOPY-IMAGING FACILITY

描述(由申请人提供)：行政和科学核心作为 为个别项目提供服务的中央单位。核心的行政部分负责管理方案的财务，包括赠款准备、非竞争性续签和报告、组织方案活动，包括研讨会、期刊俱乐部和咨询委员会的磋商，以及编写与方案有关的出版物。核心的组织培养部分以高效、协调和节省成本的方式提供集中的培养基准备，提供常见的细胞培养，并储备细胞系和一些单抗。显微镜/成像设备为研究人员提供了从凝胶(DNA或蛋白质)中捕获图像的机会，其中内置了溴化乙锭、SYBR绿、SYPRO橙和考马斯西蓝的参数。一旦图像被捕获，就可以进行调整、分析或打印。这个设施(300平方英尺)的物理部件包括一个具有荧光功能的倒置显微镜、光学元件和滤光片、一个z聚焦马达、3芯片视频系统以及提供宏编辑和3D处理的软件。在下一个项目期间，将为项目调查人员开发组织准备/现场设施。这些技术越来越多地被项目I、II和III使用，每个调查员目前都在与自己的实验室相距较远的地方借用其他调查员的设备。因为所有的项目都使用胚胎鸡肉组织来表达 在原位杂交分析中，研究人员共享了一个鸡特异性cDNA文库；其中大多数是在他们自己的实验室构建的。将根据需要继续努力进一步扩大核心的这一组成部分。

DESCRIPTION (provided by applicant): The Administrative and Scientific Core functions as a centralized unit to provide services to individual projects. The administrative component of the core is responsible for managing the finances of the program, including grant preparation, non competing renewals and reports, organizing program activities, including seminars, journal clubs and the Advisory Board consultations, and preparation of publications relevant to the program. The tissue culture component of the core provides centralized media preparation, supplies common cell cultures, and stock cell lines and some monoclonal antibodies in an efficient, coordinated and cost-saving manner. The microscopy/imaging facility provides investigators the opportunity to capture images from gels (DNA or protein) with built-in parameters for ethidium bromide, SYBR green, SYPRO orange, and Coomassie blue. Once the image has been captured it is available for adjustment, analysis or printing. The physical components in this facility (300 square feet) include an inverted microscope with fluorescence capabilities, optics and filters, a z-focus motor, 3-chip video system, and software that provides for macro editing and 3D processing. Over the next project period a tissue preparation/in situ facility will be developed for program investigators. These techniques are increasingly being used by Projects I, II and III, and each investigator is currently borrowing time on other investigator's equipment at a distance from their own laboratories. Because all projects use embryonic chick tissue for mRNA in situ hybridization analysis, a library of chick specific cDNAs is being shared among the investigators; most of them were constructed in their own laboratories. A continuous effort will be made for further expansion of this component of the core as needed.