Pilot Project

乙醇研究的主要挑战之一是确定影响乙醇的保守遗传机制 酒精对行为的影响。在小鼠和人类中进行的综合分子研究 我实验室的初步遗传分析表明，果蝇模型非常适合于识别 保守的酒精行为基因。在这个试点项目中，我们将通过以下方式扩展我们的前期工作 利用果蝇模型评估几个候选基因座对运动行为的影响 乙醇的存在。我们将要研究的所有候选基因都是小鼠和 人类基因基于初级氨基酸序列和存在的保守蛋白质结构域。在目标1中 我们将评估多个候选基因在酒精敏感性中的作用。在目标2中，我们将确定 这些候选基因是否会影响酒精耐受性。在这两个目标中，我们将探索其他 与影响酒精敏感性或耐受性的基因座协同工作的候选基因。计划中的 试点项目是利用果蝇模型识别保守遗传机制的关键一步 潜在的酒精行为。

One of the major challenges in ethanol research is to identify conserved genetic mechanisms that influence the effects of ethanol on behavior. Integrated molecular studies in mice and humans in conjunction with preliminary genetic analyses in my lab indicate that the Drosophila model is well suited for identifying conserved genes underlying ethanol behavior. In this pilot project we will expand on our preliminary work by exploiting the Drosophila model to evaluate several candidate loci for their impact on locomotor behavior in the presence of ethanol. All of the candidate genes we will investigate are good orthologues of mouse and human genes based on primary amino acid sequence and presence of conserved protein domains. In Aim 1 we will assess the role of multiple candidate genes in ethanol sensitivity. In Aim 2, we will determine whether these candidate genes influence ethanol tolerance. In both Aims, we will explore additional candidate genes that work in concert with loci that influence ethanol sensitivity or tolerance. The planned pilot project is a key step forward in using the Drosophila model to identify conserved genetic mechanisms underlying ethanol behavior.