Research Resources and Workforce Development for the Rocky Mountain Regional Biocontainment Laboratory at Colorado State University

项目概要：位于科罗拉多州的落基山地区生物防护实验室（RMRBL） 大学（CSU）一直响应国家RBL使命：1）“进行生物防御研究， 新出现的传染病病原体”; 2）“随时准备协助国家、州和地方公众 自其开放和全面实施以来， 2008年投产。RMRBL的研究人员及其合作者迅速转向应对 严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）的出现， 治疗，疫苗，并确定其他潜在的人畜共患病水库作为野生动物的溢出机会 和家畜。我们的研究人员在过去的一年里也同样致力于研究对策， 应对Mpox的重新出现，同时也继续解决高后果病原体， 长期困扰着公共卫生系统，如结核分枝杆菌， 结核除了我们已经证明的快速满足研究和研究服务需求的能力之外， 我们的团队通过以下方式为培训、外展和进入我们的设施做出贡献： 赞助奖学金、访问学者计划、讲习班、会议，并通过合作。 尽管取得了这些成绩，我们的RMRBL BSL 3套房老化，需要不断投资，以保持安全， 安全且符合BSL 3标准的设施。RMRBL BSL 3研究人员，支持人员和生物安全专业人员 易受工作环境压力、资源有限、资金缺口和参与机会的影响 在风险较低的领域配备尖端技术。在这个应用程序中，我们应对挑战 面对RMRBL BSL 3实验室，有3个核心。核心1包括改进的管理结构， 有系统地更换报废的科学仪器，并进行全面和主动的维护 现有设施需要确保合规和持续运作。RMRBL BSL 3的操作是 进一步加强，以改善工作环境，提高一致性的业务研究 支持和畜牧业工作人员。核心2的倡议寻求制定培训方案， 学习和教育最佳做法，确保提高工作人员的安全和安全合规性 BSL 3。生物安保升级将改善安全和安保环境， 标准，以及研究团队和生物安全办公室在构建和培训 技术标准作业程序将加快安全性能和技术能力。最后， 核心3综合了我们的研究优势，以开发一个独特的合格的生物遏制研究 核心资源，汇集机会，利用团队人才和进行创新研究。 结合起来，我们的三个核心确保RMRBL BSL 3设施始终“温暖准备”-面对和 对抗下一个致病性大流行的威胁。

Project Summary: The Rocky Mountain Regional Biocontainment Laboratory (RMRBL) at Colorado State University (CSU) has been responsive to the national RBL mission to: 1) “Conduct research on biodefense and emerging infectious disease agents”; 2) “Be available and prepared to assist national, state, and local public health efforts in the event of a bioterrorism or infectious disease emergency” since its opening and full commissioning in 2008. Researchers at the RMRBL and their collaborators rapidly pivoted in response to the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), working on host-derived therapeutics, vaccines, and identifying other potential zoonotic reservoirs as spillover opportunities in wildlife and domestic animals. Our researchers similarly pivoted this past year to work on countermeasures in response to the re-emergence of Mpox, while also continuing to address high consequence pathogens that have chronically plagued public health systems, such as Mycobacterium tuberculosis, the causative agent of Tuberculosis. Beyond our demonstrated ability to rapidly address research and research service needs on pathogens with pandemic potential, our team contributes to training, outreach, and access of our facility via sponsored fellowships, visiting scholars’ programs, workshops, conferences, and through collaborations. Despite these gains, our RMRBL BSL3 suites are aged, requiring constant investments to maintain safe, secure and compliant BSL3 facilities. RMRBL BSL3 researchers, support staff, and biosafety professionals are vulnerable to the strain of the work environment, limited resources, funding gaps, and opportunities to engage in less risky fields equipped with cutting edge technologies. In this application, we respond to the challenges facing the RMRBL BSL3 laboratories with 3 Cores. Core 1 includes an improved management structure, systematic replacement of deprecating scientific instruments, and comprehensive and proactive maintenance of existing facilities needs to ensure compliance and continuous functioning. Operation of the RMRBL BSL3 is additionally enhanced to improve the working environment and increase consistency for operations research support and animal husbandry staff. Core 2’s initiatives seek to develop training programs responsive to adult learning and education best practices, ensuring improved safety and safety compliance in persons working in the BSL3. Biosecurity upgrades will improve the safety and security climate in anticipation of new national standards, and integration between research teams and the office of Biosafety in constructing and training in technical standard operating procedures will accelerate safe performance and technical competence. Finally, Core 3 synthesizes our research strengths to develop a uniquely qualified Biocontainment Research Resources Core, bringing together opportunities to exploit team talent and perform innovative research. Combined, our three Cores ensure that the RMRBL BSL3 facilities are always ‘warm ready’ – to face and combat the next pathogenic pandemic threat.