Nutrient-dependent regulation of neural stem cell proliferation and neural circuit formation

项目摘要 这个夏季研究项目的目标是识别调节神经重新激活的基因。 使用模式生物黑腹果蝇从发育停滞中提取干细胞。 静息状态是干细胞处于有丝分裂休眠状态的一种重要的基本状态。 有丝分裂休眠保护干细胞免受可能导致早熟的不受控制的增殖 干细胞库耗尽或肿瘤形成。识别调控神经干细胞的基因 从静止状态重新激活，将对近亲交配的野生型苍蝇进行筛选，其基因组 已经进行了测序。然后在每一条生产线上通过喂食来检测从静止状态重新激活 检测动物EDU和增殖的神经干细胞数量。全基因组的关联 然后将进行研究，并生成候选基因列表。下一步将包括 筛选候选基因列表。该项目旨在为一名本科生提供培训 学生在暑期的几个月里。该项目将为学生提供技术培训 (研究模型生物、显微镜和基因组序列分析)和非技术领域， 职业发展活动，包括数据展示和写作技能的发展。

Project Summary The goal of this summer research project is to identify genes that regulate the reactivation of neural stem cells from developmental quiescence using the model organism Drosophila melanogaster. Quiescence is a fundamentally important stem cell state in which stem cells are mitotically dormant. Mitotic dormancy protects stem cells from uncontrolled proliferation which could lead to premature depletion of the stem cell pool or tumorigenesis. To identify genes that regulate neural stem cell reactivation from quiescence, a collection of inbred wildtype flies will be screened whose genomes have been sequenced. Reactivation from quiescence will then be assayed in each line by feeding animals EdU and numbers of proliferating neural stem cells assayed. A genome wide association study will then be carried out and a list of candidate genes will be generated. Next steps will include screening the candidate gene list. This project is aimed at providing training for one undergraduate student during the summer months. This project will provide the student both technical training (working with model organisms, microscopy, and analysis of genome sequences) and non-technical, career development activities, including data presentation and development of writing skills.