Loss of Complement-Protective CD55 Expression in Endometriosis

描述（由申请人提供）：早在1927年，Sampson就提出了子宫内膜异位症的理论，即子宫内膜组织通过逆行月经机械转移到腹膜的结果，这是大多数女性都会发生的一个过程。通过体内观察和体外研究，我们的实验室已经证明，在子宫内膜异位症的子宫内膜中，炎症样微环境改变了孕酮受体（PR-A和PR-B）的表达比例，破坏了孕酮上调转化生长因子（TGF-β）表达和下调细胞特异性基质金属蛋白酶（MMP）表达的能力。许多研究者现在怀疑子宫内膜异位症的发生是孕激素未能控制月经前迁移到子宫内膜的免疫细胞的促炎活性的可预测结果。在正常子宫内膜中，衰变加速因子（CD 55）在孕酮的作用下上调，这表明这种补体保护蛋白可能在妊娠准备期间控制免疫细胞的行为中发挥关键作用。虽然上皮CD 55表达减少与子宫内膜异位症发展的关系尚不清楚，但已知TGF-β和CD 55在炎症期间影响多形核中性粒细胞（PMN）的活化和功能。中性粒细胞是早期炎症的关键触发细胞，在月经期间可以显著增加MMP的表达。我们已经注意到，在我们使用免疫缺陷小鼠的子宫内膜异位症嵌合模型中，在人类细胞生长的异位部位，中性粒细胞迅速积累。在本申请中，我们提出子宫内膜异位症患者子宫内膜间质细胞中PR-B表达的降低将抑制TGF-β 2和CD 55在体内、体外和子宫内膜异位症实验小鼠模型中子宫内膜细胞生长的异位部位的孕酮依赖性表达。我们还预测，这些因素的表达改变将影响中性粒细胞的激活状态，迁移到异位部位的人子宫内膜细胞和组织生长的小鼠。我们的具体目标是：1）-检查子宫内膜对孕酮的反应性降低是否影响患有子宫内膜异位症的妇女的在位子宫内膜中上皮细胞的体内和体外CD 55表达; 2）-利用已建立的体外和体内共-培养模型，以检查对孕酮的敏感性降低是否影响从子宫内膜异位症患者获得的子宫内膜间质细胞介导邻近组织中CD 55表达的能力。上皮细胞，和3）-利用新建立的体内模型，以检查是否TGF-β 2和CD 55的表达与PMN募集和功能在异位部位的人细胞和组织生长在RAG 2？(c)小鼠

DESCRIPTION (provided by applicant): As early as 1927, Sampson theorized that endometriosis occurs as a consequence of the mechanical transfer of endometrial tissue to the peritoneum via retrograde menstruation, a process that occurs in most women. Using both in vivo observations and in vitro studies, our laboratory has demonstrated that, in the endometrium of endometriosis, an inflammatory-like microenvironment alters the expression ratio of progesterone receptors (PR-A and PR-B), disrupts the ability of progesterone to up-regulate transforming growth factor ¿ (TGF-¿) expression and down-regulates cell-specific matrix metalloproteinase (MMP) expression. Many investigators now suspect that endometriosis occurs as a predictable consequence of the failure of progesterone to control the proinflammatory activity of immune cells that migrate into the endometrium prior to menstruation. In the normal endometrium, decay accelerating factor (CD55) is upregulated in response to progesterone, suggesting that this complement protective protein may play a critical role in controlling the behavior of immune cells during preparation for pregnancy. Although the relationship of reduced epithelial CD55 expression to the development of endometriosis is unknown, both TGF-¿ and CD55 are known to impact the activation and function of polymorphonuclear neutrophils (PMNs) during inflammation. PMNs are key trigger cells during early inflammation and can significantly increase MMP expression during menstruation. We have noted the rapid accumulation of PMNs at ectopic sites of human cell growth in our chimeric models of endometriosis using immune deficient mice. In this application, we propose that reduced expression of PR-B in endometrial stromal cells of endometriosis patients will inhibit progesterone-dependent expression of TGF-¿2 and CD55 in vivo, in vitro and at ectopic sites of endometrial cell growth in experimental murine models of endometriosis. We also predict that altered expression of these factors will affect the activated state of PMNs that migrate into ectopic sites of human endometrial cell and tissue growth in mice. Our specific aims are: 1)- to examine whether reduced endometrial responsiveness to progesterone affects the in vivo and in vitro expression of CD55 by epithelial cells in the eutopic endometrium of women with endometriosis; 2)- to utilize established in vitro and in vivo co-culture models to examine whether reduced sensitivity to progesterone affects the ability of endometrial stromal cells acquired from endometriosis patients to mediate CD55 expression in adjacent epithelial cells, and 3)- to utilize newly established in vivo models to examine whether the expression of TGF-¿2 and CD55 correlates with PMN recruitment and function at ectopic sites of human cell and tissue growth in RAG2? (c) mice.