Flow Cytometry Core

对于需要高通量分析单个细胞的研究人员来说，流式细胞术已成为必不可少的工具 细胞。在病毒感染过程中和感染后评估人类T细胞的表型和功能是一项 所有CTRHIB项目(研究项目1-4和技术开发项目1)的关键要素。这个 CTRHIB Flow Cytotics Core将提供流式细胞仪服务，以支持人类免疫学研究 由CTRHIB调查人员。流式细胞术核心将为CTRHIB调查人员提供访问 先进的FACS ARIA流式细胞仪，可对细胞进行分析和分类。目前我们能够 在单个样品上使用11种不同的荧光染料，从而允许在 单细胞。该核心将为科学家和实验室工作人员提供操作FACS ARIA数据的培训 收集和分析。它还将为设计和分析流式细胞仪实验提供指导， 包括细胞染色、细胞分选和抗体板选择技术。

Flow cytometry has become an essential tool for investigators requiring high throughput analysis of single cells. Assessment of the phenotype and function of human T cells during and after a viral infection is a critical element to all CTRHIB projects (Research Projects 1-4 and Technology Development Project 1). The CTRHIB Flow Cytometry Core will provide flow cytometry services to support human immunology research by CTRHIB investigators. The Flow Cytometry Core will provide CTRHIB investigators with access to a state-of-the-art FACS ARIA flow cytometer to permit analysis and sorting of cells. Currently we are able to use 11 different flurochromes on a single sample thus allowing assessment of multiple parameters on a single cell. The core will provide training for scientists and laboratory staff to operate the FACS ARIA for data collection and analysis. It will also provide guidance in design and analysis of flow cytometry experiments, including techniques for cell staining, cell sorting and selection of antibody panels.