Measurement of protein associations via static light scattering

我们一直在测试怀亚特科技公司设计的系统的试生产版本，以促进成分梯度静态光散射测量。该系统基于我们实验室以前开发的概念(见前两年的年度报告)，由一个泵模块和一个软件包组成，其中泵模块包含三个注射器泵和一个在线脱气器，该软件包用于控制注射器以创建组成梯度，从光散射和浓度检测器收集数据，并在单个蛋白质的平衡自结合和两个蛋白质的异结合模型的背景下分析数据。硬件和软件是由怀亚特科技根据CRADA DK-05-0104条款借给我们的。初步结果表明，利用该系统可以在一次实验中表征和量化两个单独蛋白质物种中每一个蛋白质物种的平衡自关联和两个蛋白质的异关联，每个蛋白质只需要大约2-3 mg和不到一个小时的数据采集时间。

We have been testing pre-production versions of a system designed by Wyatt Technology Incorporated to facilitate composition-gradient static light scattering measurements. The system, based upon a concept developed previously in our laboratory (see annual reports of the previous two years), consists of a pump module containing three syringe pumps and an inline degasser, and a software package that is used to control the syringes to create composition gradients, to collect data from light scattering and concentration detectors, and to analyze the data in the context of models for equilibrium self-association of a single protein and hetero-association of two proteins. The hardware and software were loaned to us by Wyatt Technology under terms of CRADA DK-05-0104. Preliminary results indicate that with this system one can characterize and quantify equilibrium self-associations of each of two individual protein species and hetero-associations of the two proteins in a single experiment requiring only about 2-3 mg of each protein and less than one hour of data acquisition time.