发育性 CSE：对肠道细菌群落发育的影响-猫眼课题宝

发育性 CSE：对肠道细菌群落发育的影响

批准号：

9377764

负责人：

Rachel Elizabeth Neal

金额：

$45.5万

依托单位：

UNIVERSITY OF LOUISVILLE

依托单位国家：

美国

项目类别：

财政年份：

2017

资助国家：

美国

项目状态：

已结题

起止时间：

2017-08-01 至 2022-05-31

来源：

https://reporter.nih.gov/project-details/9377764

关键词：

Adult Age Animal Husbandry Animal Model Archives Bacteria Bile Acids Birth Breathing Butyrates Carbohydrates Child Cigarette Classification Coenzyme A-Transferases Communities Community Developments Consumption Cotinine DNA Data Set Development Exposure to Fatty Acids Fermentation Formate-Tetrahydrofolate Ligase Gene Expression Growth Hour Human Human Microbiome Impairment Individual Infant Inhalation Exposure Intestines Isotope Labeling Label Laboratories Libraries Life Low Birth Weight Infant Maintenance Measures Metabolic Metabolism Modeling Mothers Mus Neonatal Newborn Infant Outcome Pathogenicity Phenotype Population Pregnancy Preparation Production RNA Resistance Role Sampling Smoke Smoking Stable Isotope Labeling Starch Statistical Data Interpretation Structure Supervision Testing Tissue Banks Tobacco Tobacco smoke Toxin Training Very Low Birth Weight Infant Volatile Fatty Acids Weight World Health Organization analog carbohydrate metabolism cigarette smoking environmental tobacco smoke exposure graduate student gut microbiome maternal cigarette smoking metabolome metabolomics microbial community microbiome mouse model offspring postnatal prenatal tributyrin undergraduate student

项目摘要

项目成果

Rachel Elizabeth Neal的其他基金

相似基金

相关文献

中文摘要

翻译

摘要：香烟烟雾是最普遍的发育毒素之一。世界卫生组织估计全世界约有7亿儿童在出生前和/或出生后暴露在香烟烟雾中。这个这项建议的主要主题是研究发育期吸烟暴露对健康的影响肠道微生物群的定植、维持和功能。由于新生儿肠道定植是由分娩期间暴露于母体细菌，吸烟暴露改变盲肠细菌含量在成人中，我们认为母亲在怀孕期间吸烟会导致异常细菌新生儿肠道的定植，从而导致病原菌过度生长，并继续吸烟出生后发育期间的烟雾暴露巩固了这一表型。利用一个独特的和良好的模拟孕期和新生儿期间母亲吸烟的特征小鼠吸入模型烟雾暴露-在我们的实验室建立-我们将：1)检查发育期吸烟的影响烟雾暴露对子代肠道微生物群落定植和微生物群落持久性的影响表型；2)检测发育期香烟烟雾暴露对子代肠道微生物的影响社区功能；以及3)比较我们的发育烟草小鼠模型的结果吸烟母亲的极低出生体重儿吸烟暴露的研究结果在怀孕期间。中心假说：发育期香烟烟雾暴露导致致病碳水化合物代谢持续改变的小鼠肠道细菌定植母亲在怀孕期间吸烟对出生体重极低的婴儿也有类似的影响。

英文摘要

ABSTRACT: Cigarette smoke is one of the most prevalent developmental toxins. The World Health Organization estimates that ~700 million children worldwide are exposed either pre- and/or postnatally to cigarette smoke. The overarching theme of this proposal is to study the role of developmental cigarette smoke exposure on the colonization, maintenance, and function of the gut microbiome. Since neonatal gut colonization is initiated by exposure to maternal bacteria during birth, and tobacco smoke exposure alters cecal bacterial content in adults, we propose that maternal tobacco smoke exposure during pregnancy results in aberrant bacterial colonization of the neonatal gut such that pathogenic bacterial overgrowth occurs, and that continued cigarette smoke exposure during postnatal development entrenches this phenotype. Utilizing a unique and well characterized murine inhalation model simulating maternal cigarette smoking during pregnancy, and newborn smoke exposure - established in our laboratory - we will: 1) Examine the impact of developmental cigarette smoke exposure on offspring gut microbial community colonization and the persistence of the microbiome phenotype; 2) Examine the impact of developmental cigarette smoke exposure on offspring gut microbial community function; and 3) Compare the outcomes within our murine model of developmental tobacco cigarette smoke exposure with findings from very low birth weight human infants of mothers who smoked during pregnancy. Central Hypothesis: Developmental cigarette smoke exposure induces pathogenic intestinal bacterial colonization with persistent alterations in carbohydrate metabolism in mice, with a similar impact in very low birth weight infants of mothers who smoked during pregnancy.

期刊论文(0)

专著(0)

科研奖励(0)

会议论文