PDX Core

项目摘要-PDX Core B PDX Core为项目研究人员提供了主要资源，包括黑色素瘤PDX、黑色素瘤 细胞系和3D模型，如器官、球体和球体，以探索新的治疗方法。我们的黑色素瘤 PDX临床前平台包括500种不同的肿瘤，代表所有临床、生物和遗传学 疾病的群体。PDX核心的主要重点是建立、储存和分发新的PDX和 PDX派生的模型。PDX Core还协调临床前黑色素瘤模型的特征 在生物学(例如生长和转移)和分子(例如DNA和RNA测序以及蛋白质)方面 表达式)级别。核心维护一个主细胞库，进行样本和衍生的质量控制 老鼠病原体的模型和监测器。核心也是在内部交换PDX的中心促进者 PDXNet并协调与项目内其他调查人员的协作，整个PDXNet 以及外部科学家。该核心协调数据存储、数据共享和分发给其他调查人员 在这个项目中。 PDX核心可用于对该计划的研究人员进行所有生物分析和模型方面的培训。总的来说， 这一核心将为PDTC内的所有调查人员提供高效和高质量的服务。PDX核心是 还可根据要求协助PDXNet内的调查人员和合作者。 1

Project Summary – PDX Core B The PDX Core provides major resources to the program investigators, including melanoma PDX, melanoma cell lines and 3D models such as organoids, spheres, and spheroids, to explore new therapies. Our melanoma PDX pre-clinical platform encompasses >500 different tumors representing all clinical, biologic, and genetic groups of the disease. The primary focus of the PDX Core is to establish, bank and distribuite new PDX and PDX-derived models. The PDX Core also coordinates the characterization the pre-clinical melanoma models at at the biological (e.g. growth and metastasis) and molecular (e.g. DNA- and RNA-sequencing, and protein expression) levels. The core maintains a master cell bank, conducts quality control of specimens and derived models and monitors for mouse pathogens. The core is also the central facilitator for exchanging PDX within the PDXNet and coordinates collaborations with other investigators within the program, the overall PDXNet and outside scientists. The core coordinates data storage, data sharing and distribution to other investigators within the program. The PDX Core is available to train investigators of the program in all biological assays and models. Overall, this core will provide efficient and high-quality service to all investigators within this PDTC. The PDX Core is also available to assist investigators withinPDXNet and collaborators as requested. 1