Monkey Alcohol Tissue Research Resource (MATRR)

项目摘要 “猴子酒精组织研究资源”（MATRR）是一个多PI研究资源奖， 俄勒冈州健康与科学大学和贝勒大学。MATRR在以下方面非常成功： 为酒精研究界提供从猴子队列中产生的组织和数据， 已经完成了一项研究自愿自我饮酒的标准方案。该过程导致 广泛分布的平均每日乙醇摄入量（g/kg）和MATRR生物信息学已经建立 可靠的，强大的饮酒类别，包括低，狂欢，沉重和非常沉重的慢性摄入量 模式.尸检程序是完全完善的，并允许最高质量的收获所有器官， 体内的组织。尸检前，联系注册用户和其他相关方， 组织制备物，否则冷冻和固定的组织保留在MATRR中，直到通过 专用网络资源。所有组织都附有关于有机体和酒精摄入量变量的数据， 迅速公布调查结果。重要的是，MATRR为具有特定 在器官病理学、神经科学和基因组学方面的专业知识， 具有相似遗传学、发育过程、内分泌学、免疫学、生理学和 神经解剖学因此，MATRR是唯一的酒精研究领域，促进翻译 酒精引起的病理学机制研究的资源。MATRR资源已超过所有 在组织生成、库存控制、组织请求、组织利用、数据分析方面的期望， 产生新数据、出版物、新资助项目和新研究方向。在这次更新中，我们 建议再增加3个队列，预计将积累来自20个队列的组织和数据， 猴（总n=240（162只乙醇，78只对照））。这使得MATRR能够满足实验设计 需要大量的个体，例如遗传和体内成像数据集。库存控制 通过独特的基于网络的界面辅助，该界面还用于管理评估和作用于组织 请求。我们总共有226名注册用户，从5，590个组织中运送了3，813个组织样本 和数据请求。每一个被接受的请求都会提交一份MTA，并自动及时提交进度报告。 所请求在过去的供资中，该资源在出版物数量方面非常成功（48 在48个月内）从22个不同的实验室在13个独特的大学或研究所。仍然有很大的 进一步开发和完善这一资源的机会。我们的目标是让MATRR生物信息学 整合跨学科、器官和个人的数据，以生成关于慢性酒精使用的已知知识 紊乱最后，我们承认，除了为大规模数据分析建立关键数字外， 存在研究其他组织（如胎儿）的机会，方案的变化（如 禁欲）和额外的数据集，例如用于肝脏弹性的超声检查。

PROJECT SUMMARY The “Monkey Alcohol Tissue Research Resource” (MATRR) is a multiple PI research resource award from Oregon Health & Science University and Baylor University. The MATRR has been highly successful in providing the alcohol research community access to tissue and data generated from cohorts of monkeys that have completed a standard protocol to study voluntary alcohol self-administration. The procedure results in a wide distribution of average daily ethanol intakes (g/kg) and the MATRR bioinformatics has established reliable, robust categories of drinking that encompass low, binge, heavy and very heavy chronic intake patterns. The necropsy procedure is fully refined and allows for the highest quality harvesting of all organs and tissues in the body. Prior to necropsy, registered users and other interested parties are contacted for custom tissue preparations, otherwise frozen and fixed tissue remain in the MATRR until requests are made through a dedicated web resource. All tissues are accompanied by data on organismal and alcohol intake variables to aid in rapid publication of findings. Importantly, the MATRR provides an opportunity for laboratories with specific expertise in organ pathology, neuroscience and genomics the ability to extend their research to a primate model with similar genetics, developmental processes, endocrinology, immunology, physiology and neuroanatomy to humans. Thus, the MATRR is unique to the alcohol research field in promoting a translational resource for mechanistic studies of alcohol-induced pathologies. The MATRR resource has exceeded all expectations in terms of tissue generation, inventory control, tissue requests, tissue utilization, data analytics, novel data generated, publications, newly funded projects and new research directions. In this renewal, we propose to add 3 more cohorts, with an expected accumulation of tissues and data from 20 cohorts of monkeys (total n=240 (162 ethanol, 78 control)). This has positioned the MATRR to fill experimental designs requiring large number of individuals, for example genetic and in vivo imaging datasets. The inventory control is aided by a unique web-based interface that also serves to administratively evaluate and act upon tissue requests. In total, we have 226 registered users and have shipped over 3,813 tissue samples from 5,590 tissue and data requests. With each accepted request an MTA is filed and timely progress reports are automatically requested. In the past funding, the resource has been extremely successful in the number of publications (48 in 48 months) from 22 different laboratories at 13 unique universities or Institutes. There remain great opportunities in further building and refining this resource. Our goal is to have the MATRR bioinformatics integrate data across disciplines, organs and individuals to generate knew knowledge of chronic alcohol use disorders. Finally, we acknowledge that in addition to building critical numbers for large scale data analyses, the opportunity exists to study additional tissue (such as fetal), variations in the protocol (such as necropsy in abstinence) and additional datasets such as sonography for liver elasticity.