Center for Neural Circuits in Addiction

项目总结：总体 成瘾是一种慢性复发性大脑疾病，由神经回路的扰动引起。描绘这些 电路扰动应该提供了大量的机会，以开发新的治疗方法，预防成瘾， 治疗神经科学的新技术正在彻底改变我们测量和干预特定神经元的能力。 神经回路为了利用这些技术，这些技术应该广泛分布。我们建议开设一个 明尼苏达大学NIDA成瘾神经回路中心（UMN）进一步开发和 传播这些新技术，在成瘾神经科学方面产生开创性的工作。基于我们 集体的专业知识，我们强大的合作成瘾研究基础和我们提供的支持， 作为一个机构，我们在UMN的小组处于一个非常好的位置来组建这个中心。它将包括四个新的 研究核心：1）病毒创新核心（维克）将协助研究者应用最先进的病毒 操纵的方法，他们的研究的解剖，分子和神经回路基础的成瘾。这 Core将为定制载体的设计提供专业知识，包括关于腺相关病毒血清型组合的指导， 启动子和荧光标记; 2）结构电路核心（SCC）将提供最先进的解剖学 成瘾神经回路的映射。与大学成像中心和UMN集成 信息学研究所，SCC将提供自动化使用的大脑清理技术与中型和微型配对， CNS的规模成像; 3）行为核心成像细胞（ICBC）将提供一系列成像 在一系列空间和时间尺度上监测行为动物的大脑活动的模式。这些 形式包括光纤测光、头戴式微型显微镜（“微型镜”）和新的宽视场， 在介观和细胞水平的行为期间的视野光学成像。4)瘾 Connectome Core（ACC）将创建一个计算平台，以整合多模态功能和结构 数据来测试暴露于成瘾药物和神经连接之间的关系。该平台的可用性 应该使来自世界任何地方的外部学者能够描绘药物修饰的连接模式， 成瘾相关的生物学变量，有助于识别心理功能的生物标志物， 功能障碍我们的中心将为研究界提供：a）新技术的教育和培训; B）获得用于数据收集和分析的工具、试剂和专门知识; d）促进用户之间的新合作; e）传播研究成果 和新技术，以更广泛的成瘾研究社区。试点项目核心将促进使用 创新试点研究的核心，推动神经回路研究的信封。根据中心主任的 领导，行政核心，与专家科学顾问小组，将协调和支持 个人核心的努力。我们的目标是使该中心成为神经回路研究的国家资源 这些技术推动了高影响力的合作研究，以解决我们领域的关键知识差距。

PROJECT SUMMARY: Overall Addiction is a chronic relapsing brain disorder resulting from perturbations in neural circuits. Delineating these circuit perturbations should provide a host of opportunities to develop new therapies for addiction prevention and treatment. New technologies in neuroscience are revolutionizing our ability to measure and intervene in specified neural circuits. To take advantage, these technologies should be broadly distributed. We propose to create a NIDA Center for Neural Circuits in Addiction at the University of Minnesota (UMN) to further develop and disseminate these new techniques to produce groundbreaking work in addiction neuroscience. Based on our collective expertise, our strong base of collaborative addiction research and the support provided by our institution, our group at the UMN is in an excellent position to form this Center. It would comprise four new Research Cores: 1) The Viral Innovation Core (VIC) will assist investigators in applying state-of-the-art viral manipulation approaches to their studies of the anatomical, molecular and neural circuit bases of addiction. This Core will provide expertise for design of custom vectors, including guidance on combinations of AAV serotype, promoters, and fluorescent tags; 2) The Structural Circuits Core (SCC) will offer state-of-the-art anatomical mapping of neural circuits involved in addiction. Integrated with the University Imaging Center and UMN Informatics Institute, SCC will provide automated use of brain clearing technology paired with meso- and micro- scale imaging of the CNS; 3) The Imaging Cells during Behavior Core (ICBC) will offer a range of imaging modalities to monitor brain activity in behaving animals across a range of spatial and temporal scales. These modalities include fiber photometry, head-mounted miniature microscopes (“miniscopes”) and novel wide field- of-view optical imaging during behavior at both the mesoscopic and cellular levels. 4) The Addiction Connectome Core (ACC) will create a computational platform to integrate multimodal functional and structural data to test relationships between exposure to addictive drugs and neural connectivity. Availability of this platform should enable outside scholars from anywhere in the world to delineate drug-modified connectivity patterns and addiction-relevant biological variables, facilitating the identification of biomarkers for mental function and dysfunction. Our Center would provide to the research community: a) Education and training in new technologies; b) Access to tools, reagents and expertise for data collection and analysis; c) Further development and adoption of new technologies; d) Catalysis of new collaborations among users; and e) Dissemination of resulting research and new technologies to the wider addiction research community. The Pilot Project Core will facilitate use of the Cores for innovative pilot studies and push the envelope in neural circuit research. Under the Center Director’s leadership, the Administrative Core, with a panel of expert scientific advisors, would coordinate and support the efforts of the individual Cores. Our goal is for the Center to be a national resource for neural circuit research technologies that fuels high-impact, collaborative research to address critical knowledge gaps in our field.