Core 2: Pathology and Tissue Core

核心2 具体目标 M.D.安德森癌症中心(MDACC)白血病孢子的主要目标是改善 白血病患者的治疗。实现这一目标的一个基本组成部分是进行有重点的 涉及人体组织和血液样本的翻译研究，允许对目标生物进行调查 和正常组织，对靶组织和正常组织的治疗效果的评价，以及相关的调节 生物标志物。为此，白血病样本分配、银行和阵列分析核心(以下简称核心 2)，将收集、处理和维护患者的人体组织样本，并将这些组织分散到 白血病孢子调查员。Core还将提供全基因组的RNA和蛋白质分子图谱 表达，它提供了对细胞生理的洞察，如信号通路活动。 反洗钱组织样本、分配、银行和阵列分析核心有以下目标： 1.开发和维护血液和骨骼中完整的细胞、血清、DNA、RNA和蛋白质的储存库 骨髓标本取自MDACC的AML患者。 2.维护全面的、前瞻性的、交互式的在线数据库，提供详细的临床和病理信息 核心收到的样本的数据。 3.提供AML患者样本的反相蛋白质阵列(RPPA)，用于筛选蛋白质 在大量白血病细胞、白血病干细胞和间充质基质细胞(MSC)中表达。 4.为基因表达谱(GEP)提供RNA-Seq数据解释。 5.通过共享血液和骨髓资源，促进白血病内和外的孢子合作。

Core 2 Specific Aims The primary objective of the M.D. Anderson Cancer Center (MDACC) Leukemia Spore is to improve the treatment of patients with leukemia. A fundamental component to meeting this objective is the conduct of focused translational research involving human tissue and blood specimens, allowing investigation of the biology of target and normal tissues, evaluation of treatment effects on both target and normal tissues, and modulation of relevant biomarkers. To these ends, the Leukemia Sample Distribution, Banking and Array Profiling Core (hereafter Core 2), will collect, process and maintain human tissue specimens from patients and will disperse these tissues to Leukemia Spore investigators. The Core will also provide genome-wide molecular profiling of RNA and protein expression, which provide insights into cellular physiology such as signaling pathway activity. The AML Tissue Sample, Distribution, Banking and Array Profiling Core has the following objectives: 1. Develop and maintain a repository of intact cells, serum, DNA, RNA and protein from blood and bone marrow specimens obtained from AML patients at MDACC. 2. Maintain a comprehensive, prospective, interactive online database with detailed clinical and pathologic data for the samples received by the Core. 3. Provide Reverse Phase Protein Arrays (RPPA) from AML patient samples for screening of protein expression in bulk leukemia cells, leukemia stem cells, and mesenchymal stromal cells (MSC). 4. Provide RNA-Seq data interpretation for gene expression profiling (GEP). 5. Facilitate inter- and extra- Leukemia Spore collaborations through sharing of blood and marrow resources.