Customer Discovery for Ferrologix Digital Magnetic Sorting

项目摘要 用免疫疗法等新兴疗法治疗免疫性疾病代表着一种日益增长的 医学范例。创造有效和有针对性的治疗方法，定义潜在的免疫学 行动机制，这是至关重要的。由于免疫学的复杂性，用单细胞测量 解决问题是至关重要的，因为疾病和治疗往往是由少数细胞亚群驱动的。 然而，由于当前细胞分离和分析工具的限制，精确地映射这些复杂的 在有利于高分辨率的规模上部署单单元分辨率的交互是困难的，且成本高昂 吞吐量目标发现或简化的临床监控。最高精度的单细胞分离和 分析工具，如FAC或微流控平台，吞吐量太低，难以集成 适用于高吞吐量筛选(HTS)工作流的SBS格式。相比之下，批量细胞分选方法，如 Mac很容易集成到HTS格式中，但无法实现单细胞捕获或分析。固有的 单细胞精确度和可扩展性的权衡阻碍了免疫学研发和临床翻译工作。 通过使用I Corp技术进行的初级市场研究采访，研究人员表达了他们的观点 对目前的单细胞分离工具表示不满，称这些工具是我们工作的主要障碍 表型分析。“ 我们推断，一种可以在SBS孔板格式中集成细胞分离和单细胞分析的产品 将缓解这些瓶颈，并加快临床翻译。利用一种名为 棘轮细胞术，这项建议的目标是开发一种可以实现细胞集成的“智能平板” 纯化并排列成单独的细胞，用于SBS格式的单细胞询问。在预赛中 研究表明，棘轮细胞计量术已经证明了细胞的定量分选能力和自组装能力 将单元格转换为单个单元格格式。在第一阶段，棘轮细胞分析芯片将用于(1)纯化CD14+ 将单核细胞转化为单个细胞，然后(2)商业上可获得的磁珠的共定位 测定单个细胞的肿瘤坏死因子α分泌活性。第二阶段将重点放在棘轮细胞术的定标上 墨盒和集成到SBS井板格式的HTS工作流程。

Project Summary Treatment of immunological disorders with emerging treatments such as immunotherapy represent a growing medical paradigm. To create potent and targeted therapies, defining the underlying immunological mechanisms of action and is critical. Because of immunological complexity, measurements with single cell resolution are essential as both diseases and treatments are often driven by small subpopulations of cells. However, due to limitations with current cell isolation and analysis tools, precisely mapping these complex interactions with single cell resolution is difficult and cost prohibitive to deploy at a scale conducive for high throughput target discovery or streamlined clinical monitoring. Most high precision single cell isolation and analysis tools, such as FACS or microfluidic platforms, are too low throughput and are difficult to integrate into SBS formats for high throughput screening (HTS) workflows. Contrastingly, bulk cell sorting methods such as MACS are easily integrated into HTS formats but cannot achieve single cell capture or analysis. The inherent tradeoff of single cell precision and scalability impeads immunological R&D and clinical translation efforts. Through primary market research interviews using I Corp techniques, researchers have voiced their dissatisfaction with the current single cell isolation tools saying they are a “major roadblock in our work on phenotypic analysis.” We reasoned that a product that can integrate cell isolation and single cell analysis in an SBS well plate format would alleviate these bottlenecks and accelerate clinical translation. Leveraging a core technology known as ratcheting cytometry, the objective of this proposal to develop a “smart plate” which can achieve integrated cell purification and arrangement into individual cells for single cell interrogation in SBS format. In preliminary studies, ratcheting cytometry has demonstrated quantitative cell sorting capacity and capacity to self-assemble cells into a single cell format. In phase I, ratcheting cytometry chips will be used to (1) purify CD 14+ monocytes into individual cells followed by (2) co-localization of commercially available magnetic ELISA beads to measure TNF α secretion activity of single cells. Phase II will focus on scaling the ratcheting cytometry cartridges and integration into SBS well plate format for HTS workflows.