Imaging Core

项目摘要 耶鲁大学血液学卓越合作中心 成像核心 YCCEH的成像核心为良性血液学提供了最先进的显微镜， 用户逐年增加。在这次更新中，我们建议继续运营， 包括具有降低的光毒性的长期时间推移，用于造血的适应性超分辨率成像， 组织和自动化高通量显微镜成像1,000至10,000细胞/24小时在超 分辨率造血是一个复杂而动态的过程，涉及许多异源细胞类型。由于 由于成像数据中包含丰富的空间和/或时间信息，因此成像在以下方面具有独特的功能： 揭示了细胞动力学和组织结构对相关细胞和状态的影响。一样多 造血细胞尺寸小，粘附性最低，难以遗传操作，存在于深部， 组织，高度动态和/或极其罕见，将成像方法应用于造血的能力 研究仅限于少数几个拥有必要技术专长和仪器的实验室。 YCCEH Imaging Core旨在降低高技术门槛，促进成像应用 造血研究技术。耶鲁大学拥有许多最先进的成像设备， 由世界知名的领导者和显微镜专家监督的设施。YCCEH成像核心将使 所有研究人员都可以随时使用大量的成像平台， 造血系统，无论是本地的还是全国的。YCCEH Imaging Core将继续为其成员提供访问 最先进的仪器包括宽视野、共聚焦、双光子和流式细胞术成像。重要的是， 通过这个成像核心，我们将其作为一种服务提供， 成像模式，包括自动化长期活细胞延时显微镜，组织的光学清除， 超分辨率显微镜核心的董事Bewersdorf博士和郭博士将作为联络人， YCCEH成员和参与的设施和实验室，并将帮助确定最合适的技术， 个人研究项目。成像核心还将支持两名兼职成像专家，他们将培训 YCCEH成员，维护Bewersdorf和Guo实验室提供的仪器， 试剂、样品制备和数据分析。我们将继续推动知识的传播 通过组织专题讨论会、讲习班、研讨会和培训， 重点是学生、受训人员和其他早期调查人员。此外，成像核心将 继续开发、优化和推广适用于分析的创新报告物/探针 通过荧光成像和/或流式细胞术检测造血细胞。我们将继续鼓励青年中心 成员通过用户奖励来评估其研究项目的各种显微镜技术 通过消除将资金投资到一个不熟悉的技术领域的心理障碍， 总体而言，成像核心将促进并使个体造血研究人员能够进行观察 他们特定的造血细胞谱系，使用可以提供独特的结构，功能， 和/或动态信息。

Project Summary Yale Cooperative Center of Excellence in Hematology Imaging Core The Imaging Core of the YCCEH has provided access to state-of-the-art microscopy for benign hematology to progressively more users each year. In this renewal, we propose to continue operations with enhancements including longterm timelapse with decreased phototoxicity, adapted super resolution imaging for hematopoietic tissues and automated high-throughput microscopy for imaging of 1,000 to 10,000 cells/24 hrs at super- resolution. Hematopoiesis is a complex and dynamic process involving many heterologous cell types. Due to the rich spatial and/or temporal information contained in imaging data, imaging is uniquely powerful in revealing the influences of cellular dynamics and tissue organization on the relevant cells and states. As many hematopoietic cells are small in size, minimally adhesive, difficult to manipulate genetically, reside in deep tissues, highly dynamic and/or exceedingly rare, the ability to apply imaging approaches to hematopoiesis research has been limited to few laboratories with the requisite technical expertise and instrumentation. YCCEH Imaging Core aims to reduce the high technical hurdle and promote the application of imaging techniques for hematopoietic research. Yale is the home of many state-of-the-art imaging equipment and facilities overseen by world-renowned leaders and experts in microscopy. The YCCEH Imaging Core will make the large array of imaging platforms readily accessible to all investigators with a research interest in hematopoiesis, locally or nationally. YCCEH Imaging Core will continue to provide its members with access to state-of-the-art instrumentation including widefield, confocal, 2-photon and flow cytometry imaging. Importantly, through this Imaging Core, we are making it available as a service, access to and expertise in using innovative imaging modalities including automated long-term live-cell time-lapse microscopy, optical clearing of tissue and super-resolution microscopy. The directors of the Core, Drs. Bewersdorf and Guo will act as liaisons between YCCEH members and the participating facilities and labs and will help identifying most suitable techniques for individual research projects. The Imaging Core will also support two part time imaging specialists who will train YCCEH members, maintain the instruments provided by the Bewersdorf and Guo labs and assist with reagents, sample preparation and data analysis. We will continue to promote the dissemination of knowledge for imaging in hematopoietic research by organizing symposia, workshops, seminars and training with an emphasis on students, trainees and other early-stage investigators. Furthermore, the Imaging Core will continue to develop, optimize and disseminate innovative reporters/probes that are suitable for analyzing hematopoietic cells by fluorescence imaging and/or flow cytometry. We will continue to encourage YCCEH members to evaluate various microscopy techniques for their research projects through the user incentive program, by eliminating the psychological hurdle of investing funds into an unfamiliar technical domain. Overall, the Imaging Core will facilitate and enable individual hematopoietic researchers to make observations of their specific hematopoietic cell linages, using approaches that can provide unique structural, functional and/or dynamic information.