Microbial Genomics Biomedical Core

微生物基因组学生物医学核心(MGBC)：项目摘要/摘要 哥伦比亚大学奥布莱恩泌尿学研究中心的使命是识别遗传因素和 先天性畸形使个体易患尿路感染，如膀胱输尿管返流，以探索宿主- 细菌代谢途径，如血红素，使感染或预防感染。为了实现这一目标，微生物 基因组学和生物医学核心(MGBC)将为生物库、扩展细菌 培养、微生物基因组学(微生物组分析、比较基因组学、RNAseq)、微生物遗传学和 研究设计咨询。MGBC将使哥伦比亚奥布莱恩中心的调查人员能够 专门的基因组技术、协调的专家咨询以及改进的严密性和重复性 基因组学和精准医学。这些服务是专门为满足研究的需求而设计的 哥伦比亚奥布莱恩中心的项目。此外，MBMC将作为以下组织成员的国家资源 其他乔治·M·奥布莱恩合作研究中心和其他NIDDK泌尿科研究项目。 此外，MGBC将与行政核心密切合作，以确保遵守所有财政、 管理和资源共享策略。MGBC也将为教育的目标做出贡献 通过讲课、学生辅导和实验指导，丰富和机会池计划 机会池获奖者与微生物基因组学相关的设计。MBMC将实现这些目标 通过以下目标：1)建立来自临床明确的不同疾病患者的尿液生物储存库 尿路感染症状；2)提供微生物组和元基因组分析；3)进行扩大的尿液培养 生物鉴定；4)促进UTI优势致病克隆的全基因组测序；5)确定 通过RNAseq和实时定量聚合酶链式反应进行基因表达；6)产生细菌突变；以及7)提供 为研究项目的研究设计提供咨询和支持。

MICROBIAL GENOMICS BIOMEDICAL CORE (MGBC): PROJECT SUMMARY/ABSTRACT The mission of the Columbia University O’Brien Urology Research Center is to identify genetic factors and congenital malformations that predispose individuals to UTI, such as vesicoureteral reflux, to explore host- bacterial metabolic pathways, such as heme, that enable or prevent infection. To achieve this goal, the Microbial Genomics and Biomedical Core (MGBC) will provide high quality services for biobanking, extended bacterial culture, microbial genomics (microbiome analyses, comparative genomics, RNASeq), microbial genetics and consultation for study design. The MGBC will enable Columbia O’Brien Center investigators to have access to specialized genomic technologies, coordinated expert consultations and improved rigor and reproducibility in genomics and precision medicine. These services are specifically designed to meet the needs of the Research Projects of the Columbia O’Brien Center. Moreover, the MBMC will serve as a national resource for members of other George M. O'Brien Cooperative Research Centers and other NIDDK Urologic Research Programs. Additionally, the MGBC will work closely with the Administrative Core to ensure adherence to all fiscal, administrative, and resource sharing policies. The MGBC will also contribute to the goals of the Educational Enrichment and Opportunity Pool Programs through lectures, student mentorship, and guidance on experimental design related to microbial genomics for Opportunity Pool awardees. The MBMC will accomplish these goals through the following aims: 1) Establish a urine biorepository from clinically well-defined patients with different UTI symptoms; 2) Provide Microbiome and metagenome analyses; 3) Perform extended urine culture for organism identification; 4) Facilitate whole genome sequencing of dominant UTI pathogenic clones; 5) Determine gene expression by RNAseq and quantitative real-time PCR; 6) Generate bacterial mutants; and 7) Provide consultations and support of study design for the Research Projects.